@article{1b0e9d5f2e704fe08f9a4c67002108d6,
title = "Comparison of livestock-associated and community-associated Staphylococcus aureus pathogenicity in a mouse model of skin and soft tissue infection",
abstract = "Industrial hog operation (IHO) workers are at increased risk of carrying Staphylococcus aureus in their nares, particularly strains that are livestock-associated (LA) and multidrug-resistant. The pathogenicity of LA-S. aureus strains remains unclear, with some prior studies suggesting reduced transmission and virulence in humans compared to community-associated methicillin-resistant (CA-MRSA) S. aureus. The objective of this study was to determine the degree to which LA-S. aureus strains contracted by IHO workers cause disease relative to a representative CA-MRSA strain in a mouse model of skin and soft tissue infection (SSTI). Mice infected with CC398 LA-S. aureus strains (IHW398-1 and IHW398-2) developed larger lesion sizes with higher bacterial burden than mice infected with CA-MRSA (SF8300) (p < 0.05). The greatest lesion size and bacterial burden was seen with a CC398 strain that produced a recurrent SSTI in an IHO worker. The LA-S. aureus infected mice had decreased IL-1β protein levels compared with CA-MRSA-infected mice (p < 0.05), suggesting a suboptimal host response to LA-S. aureus SSTIs. WGSA revealed heterogeneity in virulence factor and antimicrobial resistance genes carried by LA-S. aureus and CA-MRSA strains. The observed pathogenicity suggest that more attention should be placed on preventing the spread of LA-S. aureus into human populations.",
author = "Randad, {Pranay R.} and Dillen, {Carly A.} and Ortines, {Roger V.} and David Mohr and Maliha Aziz and Price, {Lance B.} and H{\"u}lya Kaya and Jesper Larsen and Carroll, {Karen C.} and Smith, {Tara C.} and Miller, {Lloyd S.} and Heaney, {Christopher D.}",
note = "Funding Information: The authors acknowledge Nicole Kwiatkowsi and Tracy Howard (Johns Hopkins Hospital Medical Microbiology Laboratory) for assistance with microbiology procedures and sample analysis. Funding for the study was provided by National Institute for Occupational Safety and Health (NIOSH) pilot award from the Johns Hopkins NIOSH Education and Research Center grant T42OH008428, NIOSH grant K01OH010193, Award 018HEA2013 from the Sherrilyn and Ken Fisher Center for Environmental Infectious Diseases Discovery Program at the Johns Hopkins University, School of Medicine, Department of Medicine, Division of Infectious Diseases. P.R.R. was supported by NIOSH grant T42OH008428. J.L., L.B.P and M.A. were supported by the NIAID-NIH grant R01AI101371. C.D.H. was supported by NIOSH grant K01OH010193, E.W. “Al” Thrasher Award 10287, NIEHS grant R01ES026973, NIAID-NIH grant R01AI130066, and NSF grant 1316318 as part of the joint NSF-NIH-USDA Ecology and Evolution of Infectious Diseases program. L.S.M was supported by NIH grants R01AR069502, R01AR073665, and R21AI126896. T.C.S. was supported by R18 grant HS019966 from the Agency for Healthcare Research and Quality. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Funding Information: Competing Interests: L.S.M. reports grant support from MedImmune, Regeneron Pharmaceuticals, Moderna Therapeutics, and Pfizer, which are developing therapeutics and vaccines against S. aureus and other pathogens. Publisher Copyright: {\textcopyright} 2019, The Author(s).",
year = "2019",
month = dec,
day = "1",
doi = "10.1038/s41598-019-42919-y",
language = "English (US)",
volume = "9",
journal = "Scientific reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",
number = "1",
}