Comparison of in situ hybridization and monoclonal antibodies for early detection of cytomegalovirus in cell culture

J. T. McClintock; S. R. Thaker; M. Mosher; D. Jones; M. Forman; P. Charache; K. Wright; J. Keiser; F. E. Taub

doi:10.1128/jcm.27.7.1554-1559.1989

Comparison of in situ hybridization and monoclonal antibodies for early detection of cytomegalovirus in cell culture

J. T. McClintock, S. R. Thaker, M. Mosher, D. Jones, M. Forman, P. Charache, K. Wright, J. Keiser, F. E. Taub

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

The abilities of each of four diagnostic tests - direct fluorescent monoclonal antibody (direct FA) staining, indirect fluorescent monoclonal antibody (indirect FA) staining, in situ hybridization with biotinylated DNA probes, and in situ hybridization with DNA probes directly linked to enzymatically active horseradish peroxidase - to detect cytomegalovirus soon after culture were compared. Only the indirect FA test and the in situ hybridization method with directly linked HRP-DNA probes provided consistent and reliable cytomegalovirus detection as early as 15 h postinfection.

Original language	English (US)
Pages (from-to)	1554-1559
Number of pages	6
Journal	Journal of clinical microbiology
Volume	27
Issue number	7
DOIs	https://doi.org/10.1128/jcm.27.7.1554-1559.1989
State	Published - 1989
Externally published	Yes

ASJC Scopus subject areas

Microbiology (medical)

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10.1128/jcm.27.7.1554-1559.1989

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title = "Comparison of in situ hybridization and monoclonal antibodies for early detection of cytomegalovirus in cell culture",

abstract = "The abilities of each of four diagnostic tests - direct fluorescent monoclonal antibody (direct FA) staining, indirect fluorescent monoclonal antibody (indirect FA) staining, in situ hybridization with biotinylated DNA probes, and in situ hybridization with DNA probes directly linked to enzymatically active horseradish peroxidase - to detect cytomegalovirus soon after culture were compared. Only the indirect FA test and the in situ hybridization method with directly linked HRP-DNA probes provided consistent and reliable cytomegalovirus detection as early as 15 h postinfection.",

author = "McClintock, {J. T.} and Thaker, {S. R.} and M. Mosher and D. Jones and M. Forman and P. Charache and K. Wright and J. Keiser and Taub, {F. E.}",

year = "1989",

doi = "10.1128/jcm.27.7.1554-1559.1989",

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T1 - Comparison of in situ hybridization and monoclonal antibodies for early detection of cytomegalovirus in cell culture

AU - McClintock, J. T.

AU - Thaker, S. R.

AU - Mosher, M.

AU - Jones, D.

AU - Forman, M.

AU - Charache, P.

AU - Wright, K.

AU - Keiser, J.

AU - Taub, F. E.

PY - 1989

Y1 - 1989

N2 - The abilities of each of four diagnostic tests - direct fluorescent monoclonal antibody (direct FA) staining, indirect fluorescent monoclonal antibody (indirect FA) staining, in situ hybridization with biotinylated DNA probes, and in situ hybridization with DNA probes directly linked to enzymatically active horseradish peroxidase - to detect cytomegalovirus soon after culture were compared. Only the indirect FA test and the in situ hybridization method with directly linked HRP-DNA probes provided consistent and reliable cytomegalovirus detection as early as 15 h postinfection.

AB - The abilities of each of four diagnostic tests - direct fluorescent monoclonal antibody (direct FA) staining, indirect fluorescent monoclonal antibody (indirect FA) staining, in situ hybridization with biotinylated DNA probes, and in situ hybridization with DNA probes directly linked to enzymatically active horseradish peroxidase - to detect cytomegalovirus soon after culture were compared. Only the indirect FA test and the in situ hybridization method with directly linked HRP-DNA probes provided consistent and reliable cytomegalovirus detection as early as 15 h postinfection.

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DO - 10.1128/jcm.27.7.1554-1559.1989

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AN - SCOPUS:0024411837

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JO - Journal of clinical microbiology

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ER -

Comparison of in situ hybridization and monoclonal antibodies for early detection of cytomegalovirus in cell culture

Abstract

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