Comparison of a receptor binding assay with a radioimmunoassay for measuring human epidermal growth factor-urogastrone in urine

Ebba Nexø; S. I. Lamberg; Morley D. Hollenberg

doi:10.3109/00365518109090501

Comparison of a receptor binding assay with a radioimmunoassay for measuring human epidermal growth factor-urogastrone in urine

Ebba Nexø, S. I. Lamberg, Morley D. Hollenberg

Research output: Contribution to journal › Article › peer-review

31 Scopus citations

Abstract

A radioreceptor assay for human epidermal growth factor-urogastrone (EGF-URO) employing mouse EGF-URO as tracer and calibration standards and human placental receptor as binding protein is compared with a radioimmunoassay for human EGF-URO. Results employing the receptor assay indicated similar values for urinary concentrations (1 - 16nmol/l)for healthy control subjects and for patients with psoriasis. Lower values were obtained for bed-ridden patients with burns or patients recovering from orthopaedic surgery. The results obtained by radioimmunoassay were somewhat higher and correlated poorly (r = 0.5) with those obtained with the radioreceptor assay. The reason for this discrepancy is discussed.

Original language	English (US)
Pages (from-to)	577-582
Number of pages	6
Journal	Scandinavian Journal of Clinical and Laboratory Investigation
Volume	41
Issue number	6
DOIs	https://doi.org/10.3109/00365518109090501
State	Published - 1981
Externally published	Yes

Keywords

Burns
Epidermal growth factor
Psoriasis
Radioimmunoassay
Radioreceptor assay
Urinary level of EGF-URO
Urogastrone

ASJC Scopus subject areas

Clinical Biochemistry

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10.3109/00365518109090501

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@article{758665e5ae5d4346aa764fc910b8f1fd,

title = "Comparison of a receptor binding assay with a radioimmunoassay for measuring human epidermal growth factor-urogastrone in urine",

abstract = "A radioreceptor assay for human epidermal growth factor-urogastrone (EGF-URO) employing mouse EGF-URO as tracer and calibration standards and human placental receptor as binding protein is compared with a radioimmunoassay for human EGF-URO. Results employing the receptor assay indicated similar values for urinary concentrations (1 - 16nmol/l)for healthy control subjects and for patients with psoriasis. Lower values were obtained for bed-ridden patients with burns or patients recovering from orthopaedic surgery. The results obtained by radioimmunoassay were somewhat higher and correlated poorly (r = 0.5) with those obtained with the radioreceptor assay. The reason for this discrepancy is discussed.",

keywords = "Burns, Epidermal growth factor, Psoriasis, Radioimmunoassay, Radioreceptor assay, Urinary level of EGF-URO, Urogastrone",

author = "Ebba Nex{\o} and Lamberg, {S. I.} and Hollenberg, {Morley D.}",

note = "Funding Information: These studies were supported, in part, by grants from the March of Dimes Birth Defects Foundation (1-677) and the U.S. National Institutes of Health (AM 21921). During the course of these studies, M.D.H. was an Investigator of the Howard Hughes Medical Institute.",

year = "1981",

doi = "10.3109/00365518109090501",

language = "English (US)",

volume = "41",

pages = "577--582",

journal = "Scandinavian Journal of Clinical and Laboratory Investigation",

issn = "0036-5513",

publisher = "Informa Healthcare",

number = "6",

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T1 - Comparison of a receptor binding assay with a radioimmunoassay for measuring human epidermal growth factor-urogastrone in urine

AU - Nexø, Ebba

AU - Lamberg, S. I.

AU - Hollenberg, Morley D.

N1 - Funding Information: These studies were supported, in part, by grants from the March of Dimes Birth Defects Foundation (1-677) and the U.S. National Institutes of Health (AM 21921). During the course of these studies, M.D.H. was an Investigator of the Howard Hughes Medical Institute.

PY - 1981

Y1 - 1981

N2 - A radioreceptor assay for human epidermal growth factor-urogastrone (EGF-URO) employing mouse EGF-URO as tracer and calibration standards and human placental receptor as binding protein is compared with a radioimmunoassay for human EGF-URO. Results employing the receptor assay indicated similar values for urinary concentrations (1 - 16nmol/l)for healthy control subjects and for patients with psoriasis. Lower values were obtained for bed-ridden patients with burns or patients recovering from orthopaedic surgery. The results obtained by radioimmunoassay were somewhat higher and correlated poorly (r = 0.5) with those obtained with the radioreceptor assay. The reason for this discrepancy is discussed.

AB - A radioreceptor assay for human epidermal growth factor-urogastrone (EGF-URO) employing mouse EGF-URO as tracer and calibration standards and human placental receptor as binding protein is compared with a radioimmunoassay for human EGF-URO. Results employing the receptor assay indicated similar values for urinary concentrations (1 - 16nmol/l)for healthy control subjects and for patients with psoriasis. Lower values were obtained for bed-ridden patients with burns or patients recovering from orthopaedic surgery. The results obtained by radioimmunoassay were somewhat higher and correlated poorly (r = 0.5) with those obtained with the radioreceptor assay. The reason for this discrepancy is discussed.

KW - Burns

KW - Epidermal growth factor

KW - Psoriasis

KW - Radioimmunoassay

KW - Radioreceptor assay

KW - Urinary level of EGF-URO

KW - Urogastrone

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AN - SCOPUS:0019782869

SN - 0036-5513

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EP - 582

JO - Scandinavian Journal of Clinical and Laboratory Investigation

JF - Scandinavian Journal of Clinical and Laboratory Investigation

IS - 6

ER -

Comparison of a receptor binding assay with a radioimmunoassay for measuring human epidermal growth factor-urogastrone in urine

Abstract

Keywords

ASJC Scopus subject areas

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