Combined phase-sensitive acoustic microscopy and confocal laser scanning microscopy

Albert Kamanyi; Wilfred Ngwa; Timo Betz; Reinhold Wannemacher; Wolfgang Grill

doi:10.1016/j.ultras.2006.05.030

Combined phase-sensitive acoustic microscopy and confocal laser scanning microscopy

Albert Kamanyi, Wilfred Ngwa, Timo Betz, Reinhold Wannemacher, Wolfgang Grill

Research output: Contribution to journal › Article › peer-review

Abstract

Combined phase-sensitive acoustic microscopy (PSAM) at 1.2 GHz and confocal laser scanning microscopy (CLSM) in reflection and fluorescence has been implemented and applied to polymer blend films and fluorescently labeled fibroblasts and neuronal cells in order to explore the prospects and the various contrast mechanisms of this powerful technique. Topographic contrast is available for appropriate samples from CLSM in reflection and, with significantly higher precision, from the acoustic phase images. Material contrast can be gained from acoustic amplitude V(z) graphs. In the case of the biological cells investigated, the optical and acoustic images are very different and exhibit different features of the samples.

Original language	English (US)
Pages (from-to)	e1295-e1300
Journal	Ultrasonics
Volume	44
Issue number	SUPPL.
DOIs	https://doi.org/10.1016/j.ultras.2006.05.030
State	Published - Dec 22 2006
Externally published	Yes

Keywords

Cells
Confocal laser scanning microscopy
Phase-sensitive acoustic microscopy
Polymer blend

ASJC Scopus subject areas

Acoustics and Ultrasonics

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10.1016/j.ultras.2006.05.030

Cite this

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title = "Combined phase-sensitive acoustic microscopy and confocal laser scanning microscopy",

abstract = "Combined phase-sensitive acoustic microscopy (PSAM) at 1.2 GHz and confocal laser scanning microscopy (CLSM) in reflection and fluorescence has been implemented and applied to polymer blend films and fluorescently labeled fibroblasts and neuronal cells in order to explore the prospects and the various contrast mechanisms of this powerful technique. Topographic contrast is available for appropriate samples from CLSM in reflection and, with significantly higher precision, from the acoustic phase images. Material contrast can be gained from acoustic amplitude V(z) graphs. In the case of the biological cells investigated, the optical and acoustic images are very different and exhibit different features of the samples.",

keywords = "Cells, Confocal laser scanning microscopy, Phase-sensitive acoustic microscopy, Polymer blend",

author = "Albert Kamanyi and Wilfred Ngwa and Timo Betz and Reinhold Wannemacher and Wolfgang Grill",

note = "Funding Information: We acknowledge support from the European Space Agency (ESA) for this research.",

year = "2006",

month = dec,

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doi = "10.1016/j.ultras.2006.05.030",

language = "English (US)",

volume = "44",

pages = "e1295--e1300",

journal = "Ultrasonics",

issn = "0041-624X",

publisher = "Elsevier B.V.",

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}

TY - JOUR

T1 - Combined phase-sensitive acoustic microscopy and confocal laser scanning microscopy

AU - Kamanyi, Albert

AU - Ngwa, Wilfred

AU - Betz, Timo

AU - Wannemacher, Reinhold

AU - Grill, Wolfgang

N1 - Funding Information: We acknowledge support from the European Space Agency (ESA) for this research.

PY - 2006/12/22

Y1 - 2006/12/22

N2 - Combined phase-sensitive acoustic microscopy (PSAM) at 1.2 GHz and confocal laser scanning microscopy (CLSM) in reflection and fluorescence has been implemented and applied to polymer blend films and fluorescently labeled fibroblasts and neuronal cells in order to explore the prospects and the various contrast mechanisms of this powerful technique. Topographic contrast is available for appropriate samples from CLSM in reflection and, with significantly higher precision, from the acoustic phase images. Material contrast can be gained from acoustic amplitude V(z) graphs. In the case of the biological cells investigated, the optical and acoustic images are very different and exhibit different features of the samples.

AB - Combined phase-sensitive acoustic microscopy (PSAM) at 1.2 GHz and confocal laser scanning microscopy (CLSM) in reflection and fluorescence has been implemented and applied to polymer blend films and fluorescently labeled fibroblasts and neuronal cells in order to explore the prospects and the various contrast mechanisms of this powerful technique. Topographic contrast is available for appropriate samples from CLSM in reflection and, with significantly higher precision, from the acoustic phase images. Material contrast can be gained from acoustic amplitude V(z) graphs. In the case of the biological cells investigated, the optical and acoustic images are very different and exhibit different features of the samples.

KW - Cells

KW - Confocal laser scanning microscopy

KW - Phase-sensitive acoustic microscopy

KW - Polymer blend

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VL - 44

SP - e1295-e1300

JO - Ultrasonics

JF - Ultrasonics

IS - SUPPL.

ER -

Combined phase-sensitive acoustic microscopy and confocal laser scanning microscopy

Abstract

Keywords

ASJC Scopus subject areas

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