TY - JOUR
T1 - Combined intra-tendinous injection of Platelet Rich Plasma and bevacizumab accelerates and improves healing compared to Platelet Rich Plasma in tendinosis
T2 - Comprehensive assessment on a rat model
AU - Dallaudiere, Benjamin
AU - Zurlinden, Olivier
AU - Perozziello, Anne
AU - Deschamps, Lydia
AU - Larbi, Ahmed
AU - Louedec, Liliane
AU - Pesquer, Lionel
AU - Benayoun, Yohan
AU - Silvestre, Alain
AU - Serfaty, Jean Michel
PY - 2014/7/1
Y1 - 2014/7/1
N2 - Purpose: the aim of our study was to assess the potential of combined intratendinous injection of an anti-angiogenic drug: bevacizumab (AA) and Platelet Rich Plasma (PRP) to treat tendinopathy in a murine model of patellar and Achilles tendinopathy, and to evaluate its local toxicity. Material andMaterlal and method: twenty rats (80 patellar and Achilles tendons) were used for the study. We induced tendinosis (T+) in 80 tendons (patellar=40 and Achilles=40) by injecting under ultrasonography (US) guidance Collagenase 1® (day 0 = D0). Clinical examination was performed at D3, immediately followed by either PRP and AA (AAPRPT+, n=40) or PRP (PRPT+ n=40, control) US-guided intratendinous injection. Follow-up at D6, D18 and D25 using clinical, US and histology, and comparison between the 2 groups were performed. To study AA+PRP toxicity, we looked for necrosis or rupture on the 40 AAPRPT+.Results: all AAPRPT+ showed better joint mobilization compared to PRPT+ at D6 (p=0.03), D18 (p=0.04) and D25 (p=0.02). Similar results were found regarding US and histology, with smaller collagen fiber diameters (D6, p≤0.017, D25, p≤0.015), less disorganization and fewer neovessels (D25, p=0.004) in AAPRPT+ compared to PRPT+. No AA+PRP local toxicity was discovered in histology assessment.Conclusion: our study suggests that combined injection of AA and PRP in tendinosis accelerates and improves tendon’s healing compared PRP used alone, with no local toxicity.
AB - Purpose: the aim of our study was to assess the potential of combined intratendinous injection of an anti-angiogenic drug: bevacizumab (AA) and Platelet Rich Plasma (PRP) to treat tendinopathy in a murine model of patellar and Achilles tendinopathy, and to evaluate its local toxicity. Material andMaterlal and method: twenty rats (80 patellar and Achilles tendons) were used for the study. We induced tendinosis (T+) in 80 tendons (patellar=40 and Achilles=40) by injecting under ultrasonography (US) guidance Collagenase 1® (day 0 = D0). Clinical examination was performed at D3, immediately followed by either PRP and AA (AAPRPT+, n=40) or PRP (PRPT+ n=40, control) US-guided intratendinous injection. Follow-up at D6, D18 and D25 using clinical, US and histology, and comparison between the 2 groups were performed. To study AA+PRP toxicity, we looked for necrosis or rupture on the 40 AAPRPT+.Results: all AAPRPT+ showed better joint mobilization compared to PRPT+ at D6 (p=0.03), D18 (p=0.04) and D25 (p=0.02). Similar results were found regarding US and histology, with smaller collagen fiber diameters (D6, p≤0.017, D25, p≤0.015), less disorganization and fewer neovessels (D25, p=0.004) in AAPRPT+ compared to PRPT+. No AA+PRP local toxicity was discovered in histology assessment.Conclusion: our study suggests that combined injection of AA and PRP in tendinosis accelerates and improves tendon’s healing compared PRP used alone, with no local toxicity.
KW - Anti-angiogenic
KW - PRP
KW - Rat
KW - Tendinosis
KW - Tendon
KW - US
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U2 - 10.11138/mltj/2014.4.3.351
DO - 10.11138/mltj/2014.4.3.351
M3 - Article
AN - SCOPUS:84957677739
SN - 2240-4554
VL - 4
SP - 351
EP - 356
JO - Muscles, Ligaments and Tendons Journal
JF - Muscles, Ligaments and Tendons Journal
IS - 3
ER -