TY - JOUR
T1 - Co-infection by human immunodeficiency virus type 1 (HIV-1) and human T cell leukemia virus type 1 (HTLV-1)
T2 - Does immune activation lead to a faster progression to AIDS?
AU - Gudo, Eduardo S.
AU - Bhatt, Nilesh B.
AU - Bila, Dulce R.
AU - Abreu, Celina M.
AU - Tanuri, Amílcar
AU - Savino, Wilson
AU - Silva-Barbosa, Suse D.
AU - Jani, Ilesh V.
N1 - Funding Information:
We thank the efforts of the entire field-work team in facilitating the completion of this investigation specially the staff members of the Alto Maé HIV Outpatient Clinic and the staff of the Department of Immunology at the Mozambican National Institute of Health. This work was supported by funds from the World Bank Quick Impact Fund, the Mozambican Ministry of Science and Technology, AVERT, UK. the Oswaldo Cruz Foundation, CNPq/Pro-África Program and CAPES/PEC-PG Program (Brazil).
PY - 2009/12/22
Y1 - 2009/12/22
N2 - Background: Recent data have shown that HTLV-1 is prevalent among HIV positive patients in Mozambique, although the impact of HTLV-1 infection on HIV disease progression remains controversial. Our aim was to determine the phenotypic profile of T lymphocytes subsets among Mozambican patients co-infected by HIV and HTLV-1.Methods: We enrolled 29 patients co-infected by HTLV-1 and HIV (co-infected), 59 patients mono-infected by HIV (HIV) and 16 healthy controls (HC), respectively.For phenotypic analysis, cells were stained with the following fluorochrome-labeled anti-human monoclonal antibodies CD4-APC, CD8-PerCP, CD25-PE, CD62L-FITC, CD45RA-FITC. CD45RO-PE, CD38-PE; being analysed by four-colour flow cytometry.Results: We initially found that CD4+ T cell counts were significantly higher in co-infected, as compared to HIV groups. Moreover, CD4+ T Lymphocytes from co-infected patients presented significantly higher levels of CD45RO and CD25, but lower levels of CD45RA and CD62L, strongly indicating that CD4+ T cells are more activated under HTLV-1 plus HIV co-infection.Conclusion: Our data indicate that HTLV-1/HIV co-infected patients progress with higher CD4+ T cell counts and higher levels of activation markers. In this context, it is conceivable that in co-infected individuals, these higher levels of activation may account for a faster progression to AIDS.
AB - Background: Recent data have shown that HTLV-1 is prevalent among HIV positive patients in Mozambique, although the impact of HTLV-1 infection on HIV disease progression remains controversial. Our aim was to determine the phenotypic profile of T lymphocytes subsets among Mozambican patients co-infected by HIV and HTLV-1.Methods: We enrolled 29 patients co-infected by HTLV-1 and HIV (co-infected), 59 patients mono-infected by HIV (HIV) and 16 healthy controls (HC), respectively.For phenotypic analysis, cells were stained with the following fluorochrome-labeled anti-human monoclonal antibodies CD4-APC, CD8-PerCP, CD25-PE, CD62L-FITC, CD45RA-FITC. CD45RO-PE, CD38-PE; being analysed by four-colour flow cytometry.Results: We initially found that CD4+ T cell counts were significantly higher in co-infected, as compared to HIV groups. Moreover, CD4+ T Lymphocytes from co-infected patients presented significantly higher levels of CD45RO and CD25, but lower levels of CD45RA and CD62L, strongly indicating that CD4+ T cells are more activated under HTLV-1 plus HIV co-infection.Conclusion: Our data indicate that HTLV-1/HIV co-infected patients progress with higher CD4+ T cell counts and higher levels of activation markers. In this context, it is conceivable that in co-infected individuals, these higher levels of activation may account for a faster progression to AIDS.
UR - http://www.scopus.com/inward/record.url?scp=76249132034&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=76249132034&partnerID=8YFLogxK
U2 - 10.1186/1471-2334-9-211
DO - 10.1186/1471-2334-9-211
M3 - Article
C2 - 20028500
AN - SCOPUS:76249132034
SN - 1471-2334
VL - 9
JO - BMC infectious diseases
JF - BMC infectious diseases
M1 - 211
ER -