TY - JOUR
T1 - CMV pp65 and IE-1 T cell epitopes recognized by healthy subjects
AU - Slezak, Stefanie L.
AU - Bettinotti, Maria
AU - Selleri, Silvia
AU - Adams, Sharon
AU - Marincola, Francesco M.
AU - Stroncek, David F.
PY - 2007
Y1 - 2007
N2 - Background: Adoptive immune and vaccine therapies have been used to prevent cytomegalovirus (CMV) disease in recipients of hematopoietic progenitor cell transplants, but the nature of T cell responses to CMV have not been completely characterized. Methods: Peptide pools and indi vidual peptides derived from the immune-dominant CMV proteins pp65 and IE-1 and antigen-specific, cytokine flow cytometry were used to characterize the prevalence and frequency of CD4+ and CD8+ memory T cells in 20 healthy CMV-seropositive subjects. Results: CD8+ T cell responses to pp65 were detected in 35% o f subjects and to IE-1 in 40% of subjects. CD4+ T cell responses to pp65 were detected in 50% of subjects, but none were detected to IE-1. Several new IE-1 HLA class I epitopes were identified, including 4 restricted to HLA-C antigens. One region of IE-1 spanning amino acids 300 to 327 was rich in class I epitopes. The HLA class I restrictions of IE-1 peptides were more promiscuous than those of pp65 peptides. Conclusion: Since na turally occurring CD4+ and CD8+ T cell responses to pp65 were detectable in many subjects, but only CD8+ T cell responses to IE-1 were detected, pp65 may be better than IE-1 for use in vaccine and adoptive immune therapies.
AB - Background: Adoptive immune and vaccine therapies have been used to prevent cytomegalovirus (CMV) disease in recipients of hematopoietic progenitor cell transplants, but the nature of T cell responses to CMV have not been completely characterized. Methods: Peptide pools and indi vidual peptides derived from the immune-dominant CMV proteins pp65 and IE-1 and antigen-specific, cytokine flow cytometry were used to characterize the prevalence and frequency of CD4+ and CD8+ memory T cells in 20 healthy CMV-seropositive subjects. Results: CD8+ T cell responses to pp65 were detected in 35% o f subjects and to IE-1 in 40% of subjects. CD4+ T cell responses to pp65 were detected in 50% of subjects, but none were detected to IE-1. Several new IE-1 HLA class I epitopes were identified, including 4 restricted to HLA-C antigens. One region of IE-1 spanning amino acids 300 to 327 was rich in class I epitopes. The HLA class I restrictions of IE-1 peptides were more promiscuous than those of pp65 peptides. Conclusion: Since na turally occurring CD4+ and CD8+ T cell responses to pp65 were detectable in many subjects, but only CD8+ T cell responses to IE-1 were detected, pp65 may be better than IE-1 for use in vaccine and adoptive immune therapies.
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U2 - 10.1186/1479-5876-5-17
DO - 10.1186/1479-5876-5-17
M3 - Article
C2 - 17391521
AN - SCOPUS:34247159208
SN - 1479-5876
VL - 5
JO - Journal of translational medicine
JF - Journal of translational medicine
M1 - 17
ER -