Abstract
Copines are ubiquitously expressed, phospholipid-binding proteins that have been conserved through evolution. In this paper, we report the cloning and molecular characterization of a new member of the Copine family, Copine 8. This gene has been isolated and characterized using a combination of bioinformatic and experimental approaches. Using an algorithm to cluster ESTs (expressed sequence tags) that are available through the public "GoldenPath" database, Copine 8 was initially identified as a gene predominantly expressed in prostate and testis. Cloning and molecular analysis revealed that this gene is expressed in low-levels in most tissues examined. Two different isoforms of this gene have been isolated. Strongest expression of Copine 8 mRNA is seen in the prostate, heart, and brain. Taken together, this data suggest that Copine 8 may have an important role to play in prostate regulation and development.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 842-847 |
| Number of pages | 6 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 303 |
| Issue number | 3 |
| DOIs | |
| State | Published - Apr 11 2003 |
| Externally published | Yes |
Keywords
- Cloning
- Copine
- Expression analysis
- Functional genomics
- Isoforms
- Prostate
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology