Cloning, expression, and initial characterization of a novel cytokine-like gene family

Yuan Zhu; Gang Xu; Arun Patel; Megan M. McLaughlin; Carol Silverman; Kristin A. Knecht; Sharon Sweitzer; Xiaotong Li; Peter McDonnell; Rosanna Mirabile; Dawn Zimmerman; Rogely Boyce; Lauren A. Tierney; Erding Hu; George P. Livi; Bryan A. Wolf; Sherin S. Abdel-Meguid; George D. Rose; Rejeev Aurora; Preston Hensley; Michael Briggs; Peter R. Young

doi:10.1006/geno.2002.6816

Cloning, expression, and initial characterization of a novel cytokine-like gene family

Yuan Zhu, Gang Xu, Arun Patel, Megan M. McLaughlin, Carol Silverman, Kristin A. Knecht, Sharon Sweitzer, Xiaotong Li, Peter McDonnell, Rosanna Mirabile, Dawn Zimmerman, Rogely Boyce, Lauren A. Tierney, Erding Hu, George P. Livi, Bryan A. Wolf, Sherin S. Abdel-Meguid, George D. Rose, Rejeev Aurora, Preston HensleyMichael Briggs, Peter R. Young

School of Medicine

Research output: Contribution to journal › Article › peer-review

125 Scopus citations

Abstract

We report the identification and characterization of a novel cytokine-like gene family using structure-based methods to search for novel four-helix-bundle cytokines in genomics databases. There are four genes in this family, FAM3A, FAM3B, FAM3C, and FAM3D, each encoding a protein (224-235 amino acids) with a hydrophobic leader sequence. Northern analysis indicates that FAM3B is highly expressed in pancreas, FAM3D in placenta, and FAM3A and FAM3C in almost all tissues. Immunohistochemistry showed that FAM3A is expressed prominently in the vascular endothelium, particularly capillaries. We found that FAM3A and FAM3B protein were both localized to the islets of Langerhans of the endocrine pancreas. Recombinant FAM3B protein has delayed effects on β-cell function, inhibiting basal insulin secretion from a β-cell line in a dose-dependent manner.

Original language	English (US)
Pages (from-to)	144-150
Number of pages	7
Journal	Genomics
Volume	80
Issue number	2
DOIs	https://doi.org/10.1006/geno.2002.6816
State	Published - 2002

Keywords

Capillary endothelium
FAM3A
FAM3B
FAM3C
FAM3D
Novel cytokine
Pancreatic islet

ASJC Scopus subject areas

Genetics

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Zhu, Y., Xu, G., Patel, A., McLaughlin, M. M., Silverman, C., Knecht, K. A., Sweitzer, S., Li, X., McDonnell, P., Mirabile, R., Zimmerman, D., Boyce, R., Tierney, L. A., Hu, E., Livi, G. P., Wolf, B. A., Abdel-Meguid, S. S., Rose, G. D., Aurora, R., ... Young, P. R. (2002). Cloning, expression, and initial characterization of a novel cytokine-like gene family. Genomics, 80(2), 144-150. https://doi.org/10.1006/geno.2002.6816

Zhu, Y, Xu, G, Patel, A, McLaughlin, MM, Silverman, C, Knecht, KA, Sweitzer, S, Li, X, McDonnell, P, Mirabile, R, Zimmerman, D, Boyce, R, Tierney, LA, Hu, E, Livi, GP, Wolf, BA, Abdel-Meguid, SS, Rose, GD, Aurora, R, Hensley, P, Briggs, M & Young, PR 2002, 'Cloning, expression, and initial characterization of a novel cytokine-like gene family', Genomics, vol. 80, no. 2, pp. 144-150. https://doi.org/10.1006/geno.2002.6816

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abstract = "We report the identification and characterization of a novel cytokine-like gene family using structure-based methods to search for novel four-helix-bundle cytokines in genomics databases. There are four genes in this family, FAM3A, FAM3B, FAM3C, and FAM3D, each encoding a protein (224-235 amino acids) with a hydrophobic leader sequence. Northern analysis indicates that FAM3B is highly expressed in pancreas, FAM3D in placenta, and FAM3A and FAM3C in almost all tissues. Immunohistochemistry showed that FAM3A is expressed prominently in the vascular endothelium, particularly capillaries. We found that FAM3A and FAM3B protein were both localized to the islets of Langerhans of the endocrine pancreas. Recombinant FAM3B protein has delayed effects on β-cell function, inhibiting basal insulin secretion from a β-cell line in a dose-dependent manner.",

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author = "Yuan Zhu and Gang Xu and Arun Patel and McLaughlin, {Megan M.} and Carol Silverman and Knecht, {Kristin A.} and Sharon Sweitzer and Xiaotong Li and Peter McDonnell and Rosanna Mirabile and Dawn Zimmerman and Rogely Boyce and Tierney, {Lauren A.} and Erding Hu and Livi, {George P.} and Wolf, {Bryan A.} and Abdel-Meguid, {Sherin S.} and Rose, {George D.} and Rejeev Aurora and Preston Hensley and Michael Briggs and Young, {Peter R.}",

note = "Funding Information: We thank Derk J. Bergsma and Michael T. Lotze for their support and advice on this project. This study was also supported by NIH grants DK49814 and DK43354. The Diabetes Endocrinology Research Center (DERC) and its radioimmunoassay core are supported by DK19525.",

year = "2002",

doi = "10.1006/geno.2002.6816",

language = "English (US)",

volume = "80",

pages = "144--150",

journal = "Genomics",

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AU - Zhu, Yuan

AU - Xu, Gang

AU - Patel, Arun

AU - McLaughlin, Megan M.

AU - Silverman, Carol

AU - Knecht, Kristin A.

AU - Sweitzer, Sharon

AU - Li, Xiaotong

AU - McDonnell, Peter

AU - Mirabile, Rosanna

AU - Zimmerman, Dawn

AU - Boyce, Rogely

AU - Tierney, Lauren A.

AU - Hu, Erding

AU - Livi, George P.

AU - Wolf, Bryan A.

AU - Abdel-Meguid, Sherin S.

AU - Rose, George D.

AU - Aurora, Rejeev

AU - Hensley, Preston

AU - Briggs, Michael

AU - Young, Peter R.

N1 - Funding Information: We thank Derk J. Bergsma and Michael T. Lotze for their support and advice on this project. This study was also supported by NIH grants DK49814 and DK43354. The Diabetes Endocrinology Research Center (DERC) and its radioimmunoassay core are supported by DK19525.

PY - 2002

Y1 - 2002

N2 - We report the identification and characterization of a novel cytokine-like gene family using structure-based methods to search for novel four-helix-bundle cytokines in genomics databases. There are four genes in this family, FAM3A, FAM3B, FAM3C, and FAM3D, each encoding a protein (224-235 amino acids) with a hydrophobic leader sequence. Northern analysis indicates that FAM3B is highly expressed in pancreas, FAM3D in placenta, and FAM3A and FAM3C in almost all tissues. Immunohistochemistry showed that FAM3A is expressed prominently in the vascular endothelium, particularly capillaries. We found that FAM3A and FAM3B protein were both localized to the islets of Langerhans of the endocrine pancreas. Recombinant FAM3B protein has delayed effects on β-cell function, inhibiting basal insulin secretion from a β-cell line in a dose-dependent manner.

AB - We report the identification and characterization of a novel cytokine-like gene family using structure-based methods to search for novel four-helix-bundle cytokines in genomics databases. There are four genes in this family, FAM3A, FAM3B, FAM3C, and FAM3D, each encoding a protein (224-235 amino acids) with a hydrophobic leader sequence. Northern analysis indicates that FAM3B is highly expressed in pancreas, FAM3D in placenta, and FAM3A and FAM3C in almost all tissues. Immunohistochemistry showed that FAM3A is expressed prominently in the vascular endothelium, particularly capillaries. We found that FAM3A and FAM3B protein were both localized to the islets of Langerhans of the endocrine pancreas. Recombinant FAM3B protein has delayed effects on β-cell function, inhibiting basal insulin secretion from a β-cell line in a dose-dependent manner.

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KW - FAM3B

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KW - Pancreatic islet

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Cloning, expression, and initial characterization of a novel cytokine-like gene family

Abstract

Keywords

ASJC Scopus subject areas

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