Cloning and sequencing the HinfI restriction and modification genes

Srinivasan Chandrasegaran; Keith D. Lunnen; Hamilton O. Smith; Geoffrey G. Wilson

doi:10.1016/0378-1119(88)90210-7

Cloning and sequencing the HinfI restriction and modification genes

Srinivasan Chandrasegaran, Keith D. Lunnen, Hamilton O. Smith, Geoffrey G. Wilson

School of Medicine

Research output: Contribution to journal › Article › peer-review

45 Scopus citations

Abstract

The HinfI restriction and modification genes were cloned on a 3.9-kb PstI fragment inserted into the PstI site of plasmid pBR322. Both genes are confined to an internal 2.3-kb BclI-AvaI subfragment. This subfragment was sequenced. Two large open reading frames (ORF's) are present. ORF1 codes for the methylase [predicted 359 amino acids (aa)] and ORF2 codes for the endonuclease (predicted 262 or 272 aa).

Original language	English (US)
Pages (from-to)	387-392
Number of pages	6
Journal	Gene
Volume	70
Issue number	2
DOIs	https://doi.org/10.1016/0378-1119(88)90210-7
State	Published - Oct 30 1988

Keywords

Escherichia coli
Haemophilus influenzae
methylase
plasmids pBR322 and pUC19
recombinant DNA
restriction endonuclease

ASJC Scopus subject areas

Genetics

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10.1016/0378-1119(88)90210-7

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title = "Cloning and sequencing the HinfI restriction and modification genes",

abstract = "The HinfI restriction and modification genes were cloned on a 3.9-kb PstI fragment inserted into the PstI site of plasmid pBR322. Both genes are confined to an internal 2.3-kb BclI-AvaI subfragment. This subfragment was sequenced. Two large open reading frames (ORF's) are present. ORF1 codes for the methylase [predicted 359 amino acids (aa)] and ORF2 codes for the endonuclease (predicted 262 or 272 aa).",

keywords = "Escherichia coli, Haemophilus influenzae, methylase, plasmids pBR322 and pUC19, recombinant DNA, restriction endonuclease",

author = "Srinivasan Chandrasegaran and Lunnen, {Keith D.} and Smith, {Hamilton O.} and Wilson, {Geoffrey G.}",

note = "Funding Information: This work was supported by American Cancer Society Grant No. NP-567. H.O.S. is an American Cancer Society Research Professor. S.C. has a Junior Faculty Award from the American Cancer Society and is partially supported by a Mellon Foundation Grant. We thank Rosemary Redtield and Clark Riley for assistance in preparing the figures.",

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doi = "10.1016/0378-1119(88)90210-7",

language = "English (US)",

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pages = "387--392",

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TY - JOUR

T1 - Cloning and sequencing the HinfI restriction and modification genes

AU - Chandrasegaran, Srinivasan

AU - Lunnen, Keith D.

AU - Smith, Hamilton O.

AU - Wilson, Geoffrey G.

N1 - Funding Information: This work was supported by American Cancer Society Grant No. NP-567. H.O.S. is an American Cancer Society Research Professor. S.C. has a Junior Faculty Award from the American Cancer Society and is partially supported by a Mellon Foundation Grant. We thank Rosemary Redtield and Clark Riley for assistance in preparing the figures.

PY - 1988/10/30

Y1 - 1988/10/30

N2 - The HinfI restriction and modification genes were cloned on a 3.9-kb PstI fragment inserted into the PstI site of plasmid pBR322. Both genes are confined to an internal 2.3-kb BclI-AvaI subfragment. This subfragment was sequenced. Two large open reading frames (ORF's) are present. ORF1 codes for the methylase [predicted 359 amino acids (aa)] and ORF2 codes for the endonuclease (predicted 262 or 272 aa).

AB - The HinfI restriction and modification genes were cloned on a 3.9-kb PstI fragment inserted into the PstI site of plasmid pBR322. Both genes are confined to an internal 2.3-kb BclI-AvaI subfragment. This subfragment was sequenced. Two large open reading frames (ORF's) are present. ORF1 codes for the methylase [predicted 359 amino acids (aa)] and ORF2 codes for the endonuclease (predicted 262 or 272 aa).

KW - Escherichia coli

KW - Haemophilus influenzae

KW - methylase

KW - plasmids pBR322 and pUC19

KW - recombinant DNA

KW - restriction endonuclease

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DO - 10.1016/0378-1119(88)90210-7

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AN - SCOPUS:0023816268

SN - 0378-1119

VL - 70

SP - 387

EP - 392

JO - Gene

JF - Gene

IS - 2

ER -

Cloning and sequencing the HinfI restriction and modification genes

Abstract

Keywords

ASJC Scopus subject areas

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