TY - JOUR
T1 - Cloning and linkage mapping of three polymorphic tetranucleotide (TAAA)n repeats on human chromosome 21
AU - Kalaitsidaki, Marianna
AU - Cox, Tara
AU - Chakravarti, Aravinda
AU - Antonarakis, Stylianos E.
N1 - Funding Information:
This research was supported by NIH Grants HD-24605 and HG-00373 and DOE grant FG02-89ER 60857. We thank Dr. A. Warren, Dr. H. Chen, and Dr. D. Avramopoulos for advice, and A. W. Bergen for assistance in the computer analysis.
PY - 1992/12
Y1 - 1992/12
N2 - We report the cloning, sequencing, and mapping of three short sequence repeat polymorphisms due to tetranucleotide (TAAA)n repeats from human chromosome 21. These DNA markers (D21S221, D21S225, D21S226) have been cloned from the chromosome 21-specific plasmid library of J. C. Fuscoe, C. C. Collins, D. Pinkel, and J. W. Gray (1989, Genomics 5: 100-109) and were shown to be polymorphic by polymerase chain reaction amplification and polyacrylamide gel electrophoresis. Genotypes were determined in informative CEPH pedigrees and used in linkage analysis relative to other mapped markers on human chromosome 21. One of these markers, D21S221, is closely linked to the amyloid precursor protein gene (APP), which has been implicated in the etiology of familial Alzheimer disease in some families.
AB - We report the cloning, sequencing, and mapping of three short sequence repeat polymorphisms due to tetranucleotide (TAAA)n repeats from human chromosome 21. These DNA markers (D21S221, D21S225, D21S226) have been cloned from the chromosome 21-specific plasmid library of J. C. Fuscoe, C. C. Collins, D. Pinkel, and J. W. Gray (1989, Genomics 5: 100-109) and were shown to be polymorphic by polymerase chain reaction amplification and polyacrylamide gel electrophoresis. Genotypes were determined in informative CEPH pedigrees and used in linkage analysis relative to other mapped markers on human chromosome 21. One of these markers, D21S221, is closely linked to the amyloid precursor protein gene (APP), which has been implicated in the etiology of familial Alzheimer disease in some families.
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U2 - 10.1016/S0888-7543(05)80131-4
DO - 10.1016/S0888-7543(05)80131-4
M3 - Article
C2 - 1478649
AN - SCOPUS:0027092492
SN - 0888-7543
VL - 14
SP - 1071
EP - 1075
JO - Genomics
JF - Genomics
IS - 4
ER -