Cloning and characterization of dnaE, encoding the catalytic subunit of replicative DNA polymerase III, from Vibrio cholerae strain C6706

Augusto A. Franco; Peir En Yeh; Judith A. Johnson; Eileen M. Barry; Humberto Guerra; Russell Maurer; J. Glenn Morris

doi:10.1016/0378-1119(96)00155-2

Cloning and characterization of dnaE, encoding the catalytic subunit of replicative DNA polymerase III, from Vibrio cholerae strain C6706

Augusto A. Franco, Peir En Yeh, Judith A. Johnson, Eileen M. Barry, Humberto Guerra, Russell Maurer, J. Glenn Morris

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

We report that Vibrio cholerae (Vc) contains a gene homologous to Escherichia coli dnaE, the structural gene for the a (catalytic) subunit of replicative DNA polymerase III (PolIII). Despite 24% amino acid (aa) differences in the encoded proteins, the Vc gene strongly complements an E. coli dnaE temperature sensitive (ts) mutant, indicating that all functional features essential for replication are conserved.

Original language	English (US)
Pages (from-to)	281-283
Number of pages	3
Journal	Gene
Volume	175
Issue number	1-2
DOIs	https://doi.org/10.1016/0378-1119(96)00155-2
State	Published - Oct 10 1996
Externally published	Yes

Keywords

Cholera
Escherichia coli
Recombinant DNA
Salmonella typhimurium

ASJC Scopus subject areas

Genetics

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10.1016/0378-1119(96)00155-2

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title = "Cloning and characterization of dnaE, encoding the catalytic subunit of replicative DNA polymerase III, from Vibrio cholerae strain C6706",

abstract = "We report that Vibrio cholerae (Vc) contains a gene homologous to Escherichia coli dnaE, the structural gene for the a (catalytic) subunit of replicative DNA polymerase III (PolIII). Despite 24% amino acid (aa) differences in the encoded proteins, the Vc gene strongly complements an E. coli dnaE temperature sensitive (ts) mutant, indicating that all functional features essential for replication are conserved.",

keywords = "Cholera, Escherichia coli, Recombinant DNA, Salmonella typhimurium",

author = "Franco, {Augusto A.} and Yeh, {Peir En} and Johnson, {Judith A.} and Barry, {Eileen M.} and Humberto Guerra and Russell Maurer and Morris, {J. Glenn}",

year = "1996",

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doi = "10.1016/0378-1119(96)00155-2",

language = "English (US)",

volume = "175",

pages = "281--283",

journal = "Gene",

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TY - JOUR

T1 - Cloning and characterization of dnaE, encoding the catalytic subunit of replicative DNA polymerase III, from Vibrio cholerae strain C6706

AU - Franco, Augusto A.

AU - Yeh, Peir En

AU - Johnson, Judith A.

AU - Barry, Eileen M.

AU - Guerra, Humberto

AU - Maurer, Russell

AU - Morris, J. Glenn

PY - 1996/10/10

Y1 - 1996/10/10

N2 - We report that Vibrio cholerae (Vc) contains a gene homologous to Escherichia coli dnaE, the structural gene for the a (catalytic) subunit of replicative DNA polymerase III (PolIII). Despite 24% amino acid (aa) differences in the encoded proteins, the Vc gene strongly complements an E. coli dnaE temperature sensitive (ts) mutant, indicating that all functional features essential for replication are conserved.

AB - We report that Vibrio cholerae (Vc) contains a gene homologous to Escherichia coli dnaE, the structural gene for the a (catalytic) subunit of replicative DNA polymerase III (PolIII). Despite 24% amino acid (aa) differences in the encoded proteins, the Vc gene strongly complements an E. coli dnaE temperature sensitive (ts) mutant, indicating that all functional features essential for replication are conserved.

KW - Cholera

KW - Escherichia coli

KW - Recombinant DNA

KW - Salmonella typhimurium

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DO - 10.1016/0378-1119(96)00155-2

M3 - Article

C2 - 8917113

AN - SCOPUS:0030579152

SN - 0378-1119

VL - 175

SP - 281

EP - 283

JO - Gene

JF - Gene

IS - 1-2

ER -

Cloning and characterization of dnaE, encoding the catalytic subunit of replicative DNA polymerase III, from Vibrio cholerae strain C6706

Abstract

Keywords

ASJC Scopus subject areas

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