Cloning and characterization of cDNAs coding for heavy and light chains of a monoclonal antibody (MabB23) specific for human plasma apolipoprotein B-100

Ju Won Kwak; Byung Kwon Choi; Dong Lee; Yong Koo Kang; Young Gyo Seo; Won Kyung Cho; Moon Hi Han

Cloning and characterization of cDNAs coding for heavy and light chains of a monoclonal antibody (MabB23) specific for human plasma apolipoprotein B-100

Ju Won Kwak, Byung Kwon Choi, Dong Lee, Yong Koo Kang, Young Gyo Seo, Won Kyung Cho, Moon Hi Han

Research output: Contribution to journal › Article › peer-review

Abstract

We have determined the nucleotide sequences encoding the heavy and light chains of the Fab fragment of murine monoclonal antibody MabB23 (γ2b, λ), which is specific for human plasma apolipoprotein B-100 of low-density lipoproteins. The sequence analyses revealed that the variable regions of the heavy and light chains are members of mouse heavy-chain subgroup I(B) and λ light-chain, respectively. A few unusual amino acids in the framework and constant regions of the heavy-chain were also noticed.

Original language	English (US)
Pages (from-to)	237239
Number of pages	1
Journal	Gene
Volume	169
Issue number	2
State	Published - Mar 9 1996
Externally published	Yes

Keywords

Apo B-100
Cholesterol
Fab
Hyperlipidaemia
Immunoglobulin
Low-density lipoproteins
Polymerase chain reaction
Recombinant DNA

ASJC Scopus subject areas

Genetics

Cite this

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title = "Cloning and characterization of cDNAs coding for heavy and light chains of a monoclonal antibody (MabB23) specific for human plasma apolipoprotein B-100",

abstract = "We have determined the nucleotide sequences encoding the heavy and light chains of the Fab fragment of murine monoclonal antibody MabB23 (γ2b, λ), which is specific for human plasma apolipoprotein B-100 of low-density lipoproteins. The sequence analyses revealed that the variable regions of the heavy and light chains are members of mouse heavy-chain subgroup I(B) and λ light-chain, respectively. A few unusual amino acids in the framework and constant regions of the heavy-chain were also noticed.",

keywords = "Apo B-100, Cholesterol, Fab, Hyperlipidaemia, Immunoglobulin, Low-density lipoproteins, Polymerase chain reaction, Recombinant DNA",

author = "Kwak, {Ju Won} and Choi, {Byung Kwon} and Dong Lee and Kang, {Yong Koo} and Seo, {Young Gyo} and Cho, {Won Kyung} and Han, {Moon Hi}",

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T1 - Cloning and characterization of cDNAs coding for heavy and light chains of a monoclonal antibody (MabB23) specific for human plasma apolipoprotein B-100

AU - Kwak, Ju Won

AU - Choi, Byung Kwon

AU - Lee, Dong

AU - Kang, Yong Koo

AU - Seo, Young Gyo

AU - Cho, Won Kyung

AU - Han, Moon Hi

PY - 1996/3/9

Y1 - 1996/3/9

N2 - We have determined the nucleotide sequences encoding the heavy and light chains of the Fab fragment of murine monoclonal antibody MabB23 (γ2b, λ), which is specific for human plasma apolipoprotein B-100 of low-density lipoproteins. The sequence analyses revealed that the variable regions of the heavy and light chains are members of mouse heavy-chain subgroup I(B) and λ light-chain, respectively. A few unusual amino acids in the framework and constant regions of the heavy-chain were also noticed.

AB - We have determined the nucleotide sequences encoding the heavy and light chains of the Fab fragment of murine monoclonal antibody MabB23 (γ2b, λ), which is specific for human plasma apolipoprotein B-100 of low-density lipoproteins. The sequence analyses revealed that the variable regions of the heavy and light chains are members of mouse heavy-chain subgroup I(B) and λ light-chain, respectively. A few unusual amino acids in the framework and constant regions of the heavy-chain were also noticed.

KW - Apo B-100

KW - Cholesterol

KW - Fab

KW - Hyperlipidaemia

KW - Immunoglobulin

KW - Low-density lipoproteins

KW - Polymerase chain reaction

KW - Recombinant DNA

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M3 - Article

SN - 0378-1119

VL - 169

SP - 237239

JO - Gene

JF - Gene

IS - 2

ER -

Cloning and characterization of cDNAs coding for heavy and light chains of a monoclonal antibody (MabB23) specific for human plasma apolipoprotein B-100

Abstract

Keywords

ASJC Scopus subject areas

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