TY - JOUR
T1 - Cloning and characterization of a novel human RNA binding protein gene PNO1
AU - Zhou, Guang Jin
AU - Zhang, Yue
AU - Wang, Jian
AU - Guo, Jin Hu
AU - Ni, Jun
AU - Zhong, Zhao Ming
AU - Wang, Li Qun
AU - Dang, Yong Jun
AU - Dai, Jian Feng
AU - Yu, Long
N1 - Funding Information:
We thank Dr L. Dias and N. Gaugler for revision of the English. This work was supported by the National 973 program (G1998051006) of China, 863 projects (2001AA221081) of China and the National Natural Science foundation of China (30024001,30000095).
PY - 2004/6
Y1 - 2004/6
N2 - Present work reported the cloning and characterization of a human novel RNA binding gene Partner of NO B1 (PNO1), with a length of 1637bp and a putative open reading frame of 759 bp, isolated from human kidney. It is composed of seven exons and is localized on chromosome 2p14. Western blot showed that the molecular weight of PNO1 is about 35kDa. RT-PCR results in 16 human tissues indicated that PNO1 is expressed mainly in liver, lung, spleen and kidney, slightly in thymus. testis, ovary, respectively, but not in heart, brain, skeletal muscle, placenta, pancreas, prostate, small intestine, colon and peripheral blood leukocytes. GFP fusion expression in mammalian cells exhibited its localization in the nucleus, especially in nucleoli. Subcellular localization of thirteen GFP fusion PNO1 deletion proteins showed that the region of 92-230 aa is solely responsible for its nucleolar retention, and KH domain alone is not sufficient for nucleolar retention. The PNO1 family shows significant conservation in both eukaryotes and prokaryotes.
AB - Present work reported the cloning and characterization of a human novel RNA binding gene Partner of NO B1 (PNO1), with a length of 1637bp and a putative open reading frame of 759 bp, isolated from human kidney. It is composed of seven exons and is localized on chromosome 2p14. Western blot showed that the molecular weight of PNO1 is about 35kDa. RT-PCR results in 16 human tissues indicated that PNO1 is expressed mainly in liver, lung, spleen and kidney, slightly in thymus. testis, ovary, respectively, but not in heart, brain, skeletal muscle, placenta, pancreas, prostate, small intestine, colon and peripheral blood leukocytes. GFP fusion expression in mammalian cells exhibited its localization in the nucleus, especially in nucleoli. Subcellular localization of thirteen GFP fusion PNO1 deletion proteins showed that the region of 92-230 aa is solely responsible for its nucleolar retention, and KH domain alone is not sufficient for nucleolar retention. The PNO1 family shows significant conservation in both eukaryotes and prokaryotes.
KW - Gene expression
KW - Nucleolar retention
KW - PNO1
KW - Xenopus ortholog
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U2 - 10.1080/10425170410001702159
DO - 10.1080/10425170410001702159
M3 - Article
C2 - 15497447
AN - SCOPUS:3142768981
SN - 1042-5179
VL - 15
SP - 219
EP - 224
JO - DNA Sequence - Journal of DNA Sequencing and Mapping
JF - DNA Sequence - Journal of DNA Sequencing and Mapping
IS - 3
ER -