TY - JOUR
T1 - Cloning and characterization of 13 novel transcripts and the human RGS8 gene from the 1q25 region encompassing the hereditary prostate cancer (HPC1) locus
AU - Sood, Raman
AU - Bonner, Tom I.
AU - Makalowska, Izabela
AU - Stephan, Dietrich A.
AU - Robbins, Christiane M.
AU - Connors, Tim D.
AU - Morgenbesser, Sharon D.
AU - Su, Kui
AU - Faruque, Mezbah U.
AU - Pinkett, Heather
AU - Graham, Chris
AU - Baxevanis, Andreas D.
AU - Klinger, Kathy W.
AU - Landes, Greg M.
AU - Trent, Jeffrey M.
AU - Carpten, John D.
N1 - Funding Information:
The authors thank Darryl Leja for outstanding assistance with artwork. This work was supported through the National Human Genome Research Institute Intramural Program.
PY - 2001/4/15
Y1 - 2001/4/15
N2 - The aim of this study was to develop a saturated transcript map of the region encompassing the HPC1 locus to identify the susceptibility genes involved in hereditary prostate cancer (OMIM 176807) and hyperparathyroidism-jaw tumor syndrome (OMIM 145001). We previously reported the generation of a 6-Mb BAC/PAC contig of the candidate region and employed various strategies, such as database searching, exon-trapping, direct cDNA hybridization, and sample sequencing of BACs, to identify all potential transcripts. These efforts led to the identification and precise localization on the BAC contig of 59 transcripts representing 22 known genes and 37 potential transcripts represented by ESTs and exon traps. Here we report the detailed characterization of these ESTs into full-length transcript sequences, their expression pattern in various tissues, their genomic organization, and their homology to known genes. We have also identified an Alu insertion polymorphism in the intron of one of the transcripts. Overall, data on 13 novel transcripts and the human RGS8 gene (homologue of the rat RGS8 gene) are presented in this paper. Ten of the 13 novel transcripts are expressed in prostate tissue and represent positional candidates for HPC1.
AB - The aim of this study was to develop a saturated transcript map of the region encompassing the HPC1 locus to identify the susceptibility genes involved in hereditary prostate cancer (OMIM 176807) and hyperparathyroidism-jaw tumor syndrome (OMIM 145001). We previously reported the generation of a 6-Mb BAC/PAC contig of the candidate region and employed various strategies, such as database searching, exon-trapping, direct cDNA hybridization, and sample sequencing of BACs, to identify all potential transcripts. These efforts led to the identification and precise localization on the BAC contig of 59 transcripts representing 22 known genes and 37 potential transcripts represented by ESTs and exon traps. Here we report the detailed characterization of these ESTs into full-length transcript sequences, their expression pattern in various tissues, their genomic organization, and their homology to known genes. We have also identified an Alu insertion polymorphism in the intron of one of the transcripts. Overall, data on 13 novel transcripts and the human RGS8 gene (homologue of the rat RGS8 gene) are presented in this paper. Ten of the 13 novel transcripts are expressed in prostate tissue and represent positional candidates for HPC1.
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U2 - 10.1006/geno.2001.6500
DO - 10.1006/geno.2001.6500
M3 - Article
C2 - 11318611
AN - SCOPUS:0035870763
SN - 0888-7543
VL - 73
SP - 211
EP - 222
JO - Genomics
JF - Genomics
IS - 2
ER -