@inproceedings{3c3cbdb9f023437da824afec91054e5e,
title = "Clonal culture and chemodrug assay of heterogeneous cells (PC3 prostate carcinoma cells) using microfluidic single cell array chips",
abstract = "We present a single cell assay microfluidic chip that can 1) load cells into a microwell array at single cell resolution with a high capturing efficiency, 2) grow them into homogeneous clonal colonies without cell migration between neighbouring microwells, and 3) supply cell culture media or reagents to each microwell from a perfusion gravity flow induced by pressure difference between inlet and outlet reservoirs. Using the fabricated chip, we successfully identified three clonal phenotypes in prostate cancer cells and observed the drug responsiveness varies in different subtypes.",
keywords = "Clonal culture, Microfluidic, PC3, Single cell, Subclones",
author = "Jaehoon Chung and Tom Bersano-Begey and Ken Pienta and Euisik Yoon",
year = "2009",
month = jan,
day = "1",
language = "English (US)",
isbn = "9780979806421",
series = "Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences",
publisher = "Chemical and Biological Microsystems Society",
pages = "21--23",
booktitle = "Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences",
note = "13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009 ; Conference date: 01-11-2009 Through 05-11-2009",
}