Abstract
Objectives: To validate next-generation sequencing (NGS) technology for clinical diagnosis and to determine appropriate read depth. Methods: We validated the KRAS, BRAF, and EGFR genes within the Ion AmpliSeq Cancer Hotspot Panel using the Ion Torrent Personal Genome Machine (Life Technologies, Carlsbad, CA). Results: We developed a statistical model to determine the read depth needed for a given percent tumor cellularity and number of functional genomes. Bottlenecking can result from too few input genomes. By using 16 formalin-fixed, paraffin-embedded (FFPE) cancer-free specimens and 118 cancer specimens with known mutation status, we validated the six traditional analytic performance characteristics recommended by the Next-Generation Sequencing: Standardization of Clinical Testing Working Group. Baseline noise is consistent with spontaneous and FFPE-induced C:G→T:A deamination mutations. Conclusions: Redundant bioinformatic pipelines are essential, since a single analysis pipeline gave false-negative and false-positive results. NGS is sufficiently robust for the clinical detection of gene mutations, with attention to potential artifacts.
Original language | English (US) |
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Pages (from-to) | 856-866 |
Number of pages | 11 |
Journal | American journal of clinical pathology |
Volume | 141 |
Issue number | 6 |
DOIs | |
State | Published - Jun 2014 |
Keywords
- BRAF
- Deamination
- EGFR
- KRAS
- Next-generation sequencing
- Read depth
- Validation
ASJC Scopus subject areas
- Pathology and Forensic Medicine