Clinical performance of non-radioactive assays for HIV-1 DNA amplified by the polymerase chain reaction

J. M. Wages; M. Hamdallah; A. K. Fowler; C. N. Roberts; R. R. Redfield; D. S. Burke

doi:10.1007/BF00656528

Clinical performance of non-radioactive assays for HIV-1 DNA amplified by the polymerase chain reaction

J. M. Wages, M. Hamdallah, A. K. Fowler, C. N. Roberts, R. R. Redfield, D. S. Burke

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

A major factor preventing more widespread use of polymerase chain reaction in the clinical laboratory is the lack of convenient non-radioactive probe hybridization procedures which do not sacrifice sensitivity or specificity. In this report, we describe comparisons of probes labelled with biotin, digoxygenin, alkaline phosphatase, and³²P. We report the comparison of solution or liquid hybridization assay and Southern blotting with digoxygenin-labelled oligonucleotides on a total of 64 clinical specimens. Perfect diagnostic agreement between the³²P and digoxygenin probes was obtained. These data suggest that the non-radioactive assay as described is as sensitive and as specific as the assay with³²P-Iabelled probes.

Original language	English (US)
Pages (from-to)	102-107
Number of pages	6
Journal	World Journal of Microbiology & Biotechnology
Volume	9
Issue number	1
DOIs	https://doi.org/10.1007/BF00656528
State	Published - Jan 1993
Externally published	Yes

Keywords

HIV-1
non-radioactive DNA probes
polymerase chain reaction

ASJC Scopus subject areas

Biotechnology
Physiology
Applied Microbiology and Biotechnology

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10.1007/BF00656528

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abstract = "A major factor preventing more widespread use of polymerase chain reaction in the clinical laboratory is the lack of convenient non-radioactive probe hybridization procedures which do not sacrifice sensitivity or specificity. In this report, we describe comparisons of probes labelled with biotin, digoxygenin, alkaline phosphatase, and32P. We report the comparison of solution or liquid hybridization assay and Southern blotting with digoxygenin-labelled oligonucleotides on a total of 64 clinical specimens. Perfect diagnostic agreement between the32P and digoxygenin probes was obtained. These data suggest that the non-radioactive assay as described is as sensitive and as specific as the assay with32P-Iabelled probes.",

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AU - Fowler, A. K.

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AU - Redfield, R. R.

AU - Burke, D. S.

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AB - A major factor preventing more widespread use of polymerase chain reaction in the clinical laboratory is the lack of convenient non-radioactive probe hybridization procedures which do not sacrifice sensitivity or specificity. In this report, we describe comparisons of probes labelled with biotin, digoxygenin, alkaline phosphatase, and32P. We report the comparison of solution or liquid hybridization assay and Southern blotting with digoxygenin-labelled oligonucleotides on a total of 64 clinical specimens. Perfect diagnostic agreement between the32P and digoxygenin probes was obtained. These data suggest that the non-radioactive assay as described is as sensitive and as specific as the assay with32P-Iabelled probes.

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Clinical performance of non-radioactive assays for HIV-1 DNA amplified by the polymerase chain reaction

Abstract

Keywords

ASJC Scopus subject areas

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