Clathrin assembly proteins AP180 and CALM in the embryonic rat brain

Catherine M. Schwartz, Aiwu Cheng, Mohamed R. Mughal, Mark P. Mattson, Pamela J. Yao

Research output: Contribution to journalArticlepeer-review

14 Scopus citations


Clathrin-coated vesicles are known to play diverse and pivotal roles in cells. The proper formation of clathrincoated vesicles is dependent on, and highly regulated by, a large number of clathrin assembly proteins. These assembly proteins likely determine the functional specificity of clathrin-coated vesicles, and together they control a multitude of intracellular trafficking pathways, including those involved in embryonic development. In this study, we focus on two closely related clathrin assembly proteins, AP180 and CALM (clathrin assembly lymphoid myeloid leukemia protein), in the developing embryonic rat brain. We find that AP180 begins to be expressed at embryonic day 14 (E14), but only in postmitotic cells that have acquired a neuronal fate. CALM, on the other hand, is expressed as early as E12, by both neural stem cells and postmitotic neurons. In vitro loss-of-function studies using RNA interference (RNAi) indicate that AP180 and CALM are dispensable for some aspects of embryonic neurogenesis but are required for the growth of postmitotic neurons. These results identify the developmental stage of AP180 and CALM expression and suggest that each protein has distinct functions in neural development.

Original languageEnglish (US)
Pages (from-to)3803-3818
Number of pages16
JournalJournal of Comparative Neurology
Issue number18
StatePublished - Sep 15 2010
Externally publishedYes


  • Dopaminergic
  • Intracellular trafficking
  • Neural stem cells
  • Postmitotic neurons

ASJC Scopus subject areas

  • General Neuroscience
  • General Medicine


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