Chronic kidney disease causes disruption of gastric and small intestinal epithelial tight junction

Nosratola D. Vaziri, Jun Yuan, Sohrab Nazertehrani, Zhenmin Ni, Shuman Liu

Research output: Contribution to journalArticlepeer-review

103 Scopus citations


Background: Integrity of the tight junction (TJ) which seals the gap between the epithelial cells of the gastrointestinal tract is critical in preventing the entry of the microbial toxins, antigens, and other harmful products in the subepithelial tissues and the internal milieu. By enabling the absorption of these products, impairment of the intestinal epithelial barrier leads to local and systemic inflammation. We have recently found depletion of the key protein constituents of colonic epithelial TJ in animals with chronic kidney disease (CKD). Postmortem studies have revealed the presence of inflammation throughout the gastrointestinal tract in uremic humans. This observation suggests that uremia may cause disruption of the epithelial barrier in all segments of the gastrointestinal tract including the stomach, jejunum, and ileum. The present study was undertaken to explore this possibility. Methods: Sprague-Dawley rats were randomized to CKD or control groups. The CKD group was subjected to 5/6 nephrectomy while the control group underwent a sham operation. The animals were observed for 10 weeks at which time they were euthanized and their stomachs, jejunums, and ileums were removed and processed for measurement of TJ proteins. Results: The CKD rats showed marked azotemia, systemic oxidative stress, and marked depletion of the key protein constituents of the epithelial TJ (claudin-1, occludin, and ZO1) in the stomach, jejunum, and ileum. Conclusions: The present study extends the earlier finding of uremia-induced disruption of colonic epithelial TJ by documenting the involvement of the stomach, jejunum, and ileum as well.

Original languageEnglish (US)
Pages (from-to)99-103
Number of pages5
JournalAmerican Journal of Nephrology
Issue number2
StatePublished - Aug 2013
Externally publishedYes


  • End-stage renal disease
  • Enterocolitis
  • Gastritis
  • Inflammation
  • Malnutrition
  • Uremia

ASJC Scopus subject areas

  • Nephrology


Dive into the research topics of 'Chronic kidney disease causes disruption of gastric and small intestinal epithelial tight junction'. Together they form a unique fingerprint.

Cite this