TY - JOUR
T1 - Chronic exercise decreases cytokine production in healthy rat skeletal muscle
AU - Lira, Fábio S.
AU - Koyama, Cristiane H.
AU - Yamashita, Alex S.
AU - Rosa, José C.
AU - Zanchi, Nelo E.
AU - Batista, Miguel L.
AU - Seelaender, Marília C.
N1 - Copyright:
Copyright 2011 Elsevier B.V., All rights reserved.
PY - 2009/10
Y1 - 2009/10
N2 - Skeletal muscle is the source of pro- and anti-inflammatory cytokines, and recently, it has been recognized as an important source of interleukin-6 (IL-6). Acute physical exercise is known to induce a pro-inflammatory cytokine profile in the plasma. However, the effect of chronic physical exercise in the production of pro- and anti-inflammatory cytokines by the skeletal muscle has never been examined. We assessed IL-6, TNF-α, IL-1β and IL-10 levels in the skeletal muscle of rats submitted to endurance training. Animals were randomly assigned to either a sedentary group (S, n=7) or an endurance exercise trained group (T, n=8). Trained rats ran on a treadmill for 5 days week -1 for 8 weeks (60% VO2max). Detection of IL-6, TNF-α, IL-1β and IL-10 protein expression was carried out by ELISA. We found decreased expression of IL-1β, IL-6, TNF-α and IL-10 (28%, 27%, 32% and 37%, respectively, p<0.05) in the extensor digital longus (EDL) from T, when compared with S. In the soleus, IL-1β, TNF-α and IL-10 protein levels were similarly decreased (34%, 42% and 50%, respectively, p<0.05) in T in relation to S, while IL-6 expression was not affected by the training protocol. In conclusion, exercise training induced decreased cytokine protein expression in the skeletal muscle. These data show that in healthy rats, 8-week moderate-intensity aerobic training down regulates skeletal muscle production of cytokines involved in the onset, maintenance and regulation of inflammation, and that the response is heterogeneous according to fibre composition.
AB - Skeletal muscle is the source of pro- and anti-inflammatory cytokines, and recently, it has been recognized as an important source of interleukin-6 (IL-6). Acute physical exercise is known to induce a pro-inflammatory cytokine profile in the plasma. However, the effect of chronic physical exercise in the production of pro- and anti-inflammatory cytokines by the skeletal muscle has never been examined. We assessed IL-6, TNF-α, IL-1β and IL-10 levels in the skeletal muscle of rats submitted to endurance training. Animals were randomly assigned to either a sedentary group (S, n=7) or an endurance exercise trained group (T, n=8). Trained rats ran on a treadmill for 5 days week -1 for 8 weeks (60% VO2max). Detection of IL-6, TNF-α, IL-1β and IL-10 protein expression was carried out by ELISA. We found decreased expression of IL-1β, IL-6, TNF-α and IL-10 (28%, 27%, 32% and 37%, respectively, p<0.05) in the extensor digital longus (EDL) from T, when compared with S. In the soleus, IL-1β, TNF-α and IL-10 protein levels were similarly decreased (34%, 42% and 50%, respectively, p<0.05) in T in relation to S, while IL-6 expression was not affected by the training protocol. In conclusion, exercise training induced decreased cytokine protein expression in the skeletal muscle. These data show that in healthy rats, 8-week moderate-intensity aerobic training down regulates skeletal muscle production of cytokines involved in the onset, maintenance and regulation of inflammation, and that the response is heterogeneous according to fibre composition.
KW - Endurance training
KW - Pro- and anti-inflammatory cytokines
KW - Skeletal muscle
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U2 - 10.1002/cbf.1594
DO - 10.1002/cbf.1594
M3 - Article
C2 - 19681095
AN - SCOPUS:70350582725
SN - 0263-6484
VL - 27
SP - 458
EP - 461
JO - Cell Biochemistry and Function
JF - Cell Biochemistry and Function
IS - 7
ER -