TY - JOUR
T1 - Chronic CMV infection in older women
T2 - Longitudinal comparisons of CMV DNA in peripheral monocytes, anti-CMV IgG titers, serum IL-6 levels, and CMV pp65 (NLV)-specific CD8+ T-cell frequencies with twelve year follow-up
AU - Li, Huifen
AU - Weng, Peter
AU - Najarro, Kevin
AU - Xue, Qian Li
AU - Semba, Richard D.
AU - Margolick, Joseph B.
AU - Leng, Sean X.
N1 - Funding Information:
This work is supported in part by NIH grant R21-AG-043874 (PI: Dr. Sean X. Leng) and funding from the Irma and Paul Milstein Program for Senior Health of the Milstein Medical Asian American Partnership (MMAAP) Foundation ( www.mmaapf.org ) (to Dr. Sean X. Leng).
PY - 2014/6
Y1 - 2014/6
N2 - Chronic cytomegalovirus (CMV) infection may contribute significantly to T-cell immunosenescence, chronic inflammation, and adverse health outcomes in older adults. Recent studies suggest detectable CMV DNA in peripheral monocytes as a better indicator for this persistent viral infection than anti-CMV IgG serology. Here, we conducted longitudinal comparisons of anti-CMV IgG titers, CMV DNA in the peripheral monocytes, serum IL-6 levels, and CMV pp65 (NLV)-specific CD8+ T-cell frequencies in fifteen community-dwelling older women with twelve year follow-up. The results showed that anti-CMV IgG titers did not change over twelve years. Women with detectable CMV DNA had significantly higher IL-6 levels than those without, both at baseline (3.06±0.58 vs 1.19±0.37pg/ml, respectively, p<.001) and at the follow-up (3.23±0.66 versus 0.98±0.37pg/ml, respectively, p<.001). In addition, CMV pp65 (NLV)-specific CD8+ T cells were detected only in women who had CMV DNA with similar frequencies at both time points. These findings indicate that anti-CMV IgG serology is neither sensitive to change nor useful for monitoring chronic CMV infection over time. They also provide a basis for further investigation into chronic CMV infection as defined by detectable CMV DNA in the peripheral monocytes and its impact on immunity and health in the elderly.
AB - Chronic cytomegalovirus (CMV) infection may contribute significantly to T-cell immunosenescence, chronic inflammation, and adverse health outcomes in older adults. Recent studies suggest detectable CMV DNA in peripheral monocytes as a better indicator for this persistent viral infection than anti-CMV IgG serology. Here, we conducted longitudinal comparisons of anti-CMV IgG titers, CMV DNA in the peripheral monocytes, serum IL-6 levels, and CMV pp65 (NLV)-specific CD8+ T-cell frequencies in fifteen community-dwelling older women with twelve year follow-up. The results showed that anti-CMV IgG titers did not change over twelve years. Women with detectable CMV DNA had significantly higher IL-6 levels than those without, both at baseline (3.06±0.58 vs 1.19±0.37pg/ml, respectively, p<.001) and at the follow-up (3.23±0.66 versus 0.98±0.37pg/ml, respectively, p<.001). In addition, CMV pp65 (NLV)-specific CD8+ T cells were detected only in women who had CMV DNA with similar frequencies at both time points. These findings indicate that anti-CMV IgG serology is neither sensitive to change nor useful for monitoring chronic CMV infection over time. They also provide a basis for further investigation into chronic CMV infection as defined by detectable CMV DNA in the peripheral monocytes and its impact on immunity and health in the elderly.
KW - Aging
KW - CMV DNA in monocytes
KW - Chronic CMV infection
KW - IL-6
KW - Longitudinal
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U2 - 10.1016/j.exger.2014.01.010
DO - 10.1016/j.exger.2014.01.010
M3 - Article
C2 - 24440388
AN - SCOPUS:84898775483
SN - 0531-5565
VL - 54
SP - 84
EP - 89
JO - Experimental Gerontology
JF - Experimental Gerontology
ER -