Chronic arsenic exposure impairs macrophage functions in the exposed individuals

Nilanjana Banerjee; Saptarshi Banerjee; Rupashree Sen; Apurba Bandyopadhyay; Nilendu Sarma; Papiya Majumder; Jayanta K. Das; Mitali Chatterjee; Syed N. Kabir; Ashok K. Giri

doi:10.1007/s10875-009-9304-x

Chronic arsenic exposure impairs macrophage functions in the exposed individuals

Nilanjana Banerjee, Saptarshi Banerjee, Rupashree Sen, Apurba Bandyopadhyay, Nilendu Sarma, Papiya Majumder, Jayanta K. Das, Mitali Chatterjee, Syed N. Kabir, Ashok K. Giri

Research output: Contribution to journal › Article › peer-review

64 Scopus citations

Abstract

Introduction: Owing to the established roles of human macrophages in immune defense, we investigated the effect of chronic arsenic exposure upon these major hematopoietic cells in 70 arsenic-exposed individuals with skin lesions and 64 unexposed individuals. Methods: Human monocyte-derived macrophages were prepared from peripheral blood mononuclear cells, by culture of the adherent cells for 6 days in medium supplemented with granulocyte-monocyte colony stimulating factor. Parameters studied included cell adhesion capacity, expression of CD54 and F-actin, nitric oxide production, phagocytic capacity, and effect of arsenic on Rho A-ROCK pathway. Results: In macrophages of exposed individuals when compared to unexposed group, there was cell rounding accompanied with a significant (p<0.001) loss of cell adhesion capacity, decrease in nitric oxide production, impaired phagocytic capacity, and decreased CD 54 and F-actin expression. Additionally, chronic arsenic exposure affected Rho A-ROCK pathway which in turn impaired macrophage functions. Discussion and Conclusion: These altogether could contribute significantly to arsenic-induced immunosuppression observed in the arsenic-exposed individuals.

Original language	English (US)
Pages (from-to)	582-594
Number of pages	13
Journal	Journal of Clinical Immunology
Volume	29
Issue number	5
DOIs	https://doi.org/10.1007/s10875-009-9304-x
State	Published - Sep 2009
Externally published	Yes

Keywords

Arsenic
Cell adhesion
Macrophage
Phagocytosis
Rho A-ROCK pathway

ASJC Scopus subject areas

Immunology and Allergy
Immunology

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10.1007/s10875-009-9304-x

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title = "Chronic arsenic exposure impairs macrophage functions in the exposed individuals",

abstract = "Introduction: Owing to the established roles of human macrophages in immune defense, we investigated the effect of chronic arsenic exposure upon these major hematopoietic cells in 70 arsenic-exposed individuals with skin lesions and 64 unexposed individuals. Methods: Human monocyte-derived macrophages were prepared from peripheral blood mononuclear cells, by culture of the adherent cells for 6 days in medium supplemented with granulocyte-monocyte colony stimulating factor. Parameters studied included cell adhesion capacity, expression of CD54 and F-actin, nitric oxide production, phagocytic capacity, and effect of arsenic on Rho A-ROCK pathway. Results: In macrophages of exposed individuals when compared to unexposed group, there was cell rounding accompanied with a significant (p<0.001) loss of cell adhesion capacity, decrease in nitric oxide production, impaired phagocytic capacity, and decreased CD 54 and F-actin expression. Additionally, chronic arsenic exposure affected Rho A-ROCK pathway which in turn impaired macrophage functions. Discussion and Conclusion: These altogether could contribute significantly to arsenic-induced immunosuppression observed in the arsenic-exposed individuals.",

keywords = "Arsenic, Cell adhesion, Macrophage, Phagocytosis, Rho A-ROCK pathway",

author = "Nilanjana Banerjee and Saptarshi Banerjee and Rupashree Sen and Apurba Bandyopadhyay and Nilendu Sarma and Papiya Majumder and Das, {Jayanta K.} and Mitali Chatterjee and Kabir, {Syed N.} and Giri, {Ashok K.}",

note = "Funding Information: Acknowledgments The authors are grateful to the Council of Scientific and Industrial Research (CSIR) for providing senior research fellowship to Nilanjana Banerjee and Indian Council of Medical Research (ICMR) for providing senior research fellowship to Rupashree Sen. The authors are also thankful to Fogarty International Training Program in collaboration with University of California, Berkeley, for providing training to Nilanjana Banerjee and Saptarshi Banerjee.",

year = "2009",

month = sep,

doi = "10.1007/s10875-009-9304-x",

language = "English (US)",

volume = "29",

pages = "582--594",

journal = "Journal of Clinical Immunology",

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TY - JOUR

T1 - Chronic arsenic exposure impairs macrophage functions in the exposed individuals

AU - Banerjee, Nilanjana

AU - Banerjee, Saptarshi

AU - Sen, Rupashree

AU - Bandyopadhyay, Apurba

AU - Sarma, Nilendu

AU - Majumder, Papiya

AU - Das, Jayanta K.

AU - Chatterjee, Mitali

AU - Kabir, Syed N.

AU - Giri, Ashok K.

N1 - Funding Information: Acknowledgments The authors are grateful to the Council of Scientific and Industrial Research (CSIR) for providing senior research fellowship to Nilanjana Banerjee and Indian Council of Medical Research (ICMR) for providing senior research fellowship to Rupashree Sen. The authors are also thankful to Fogarty International Training Program in collaboration with University of California, Berkeley, for providing training to Nilanjana Banerjee and Saptarshi Banerjee.

PY - 2009/9

Y1 - 2009/9

N2 - Introduction: Owing to the established roles of human macrophages in immune defense, we investigated the effect of chronic arsenic exposure upon these major hematopoietic cells in 70 arsenic-exposed individuals with skin lesions and 64 unexposed individuals. Methods: Human monocyte-derived macrophages were prepared from peripheral blood mononuclear cells, by culture of the adherent cells for 6 days in medium supplemented with granulocyte-monocyte colony stimulating factor. Parameters studied included cell adhesion capacity, expression of CD54 and F-actin, nitric oxide production, phagocytic capacity, and effect of arsenic on Rho A-ROCK pathway. Results: In macrophages of exposed individuals when compared to unexposed group, there was cell rounding accompanied with a significant (p<0.001) loss of cell adhesion capacity, decrease in nitric oxide production, impaired phagocytic capacity, and decreased CD 54 and F-actin expression. Additionally, chronic arsenic exposure affected Rho A-ROCK pathway which in turn impaired macrophage functions. Discussion and Conclusion: These altogether could contribute significantly to arsenic-induced immunosuppression observed in the arsenic-exposed individuals.

AB - Introduction: Owing to the established roles of human macrophages in immune defense, we investigated the effect of chronic arsenic exposure upon these major hematopoietic cells in 70 arsenic-exposed individuals with skin lesions and 64 unexposed individuals. Methods: Human monocyte-derived macrophages were prepared from peripheral blood mononuclear cells, by culture of the adherent cells for 6 days in medium supplemented with granulocyte-monocyte colony stimulating factor. Parameters studied included cell adhesion capacity, expression of CD54 and F-actin, nitric oxide production, phagocytic capacity, and effect of arsenic on Rho A-ROCK pathway. Results: In macrophages of exposed individuals when compared to unexposed group, there was cell rounding accompanied with a significant (p<0.001) loss of cell adhesion capacity, decrease in nitric oxide production, impaired phagocytic capacity, and decreased CD 54 and F-actin expression. Additionally, chronic arsenic exposure affected Rho A-ROCK pathway which in turn impaired macrophage functions. Discussion and Conclusion: These altogether could contribute significantly to arsenic-induced immunosuppression observed in the arsenic-exposed individuals.

KW - Arsenic

KW - Cell adhesion

KW - Macrophage

KW - Phagocytosis

KW - Rho A-ROCK pathway

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Chronic arsenic exposure impairs macrophage functions in the exposed individuals

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