Chromogranin‐a expression in gastric and colon cancer tissues

Jae‐Gahb ‐G Park, Ghee Young Choe, Lee J. Helman, Adi F. Gazdar, Han‐Kwang ‐K Yang, Jin‐Pok ‐P Kim, Seong Hoe Park, Yong Il Kim

Research output: Contribution to journalArticlepeer-review

27 Scopus citations


We studied the expression of chromogranin A (CgA) in human gastric (n = 17) and colorectal (n = 18) adenocarcinomas by nucleic acid hybridization and immunohistochemical analyses using a specific monoclonal antibody (MAb) to human chromogranin A (CgA). Some corresponding adjacent nonmalignant mucosal tissues were also examined. Results: (I) Northern blotting: of 3 normal gastric mucosas examined, 2 (67%) had an easily detected signal for expression of CgA. Only one of 14 gastric carcinomas (7%) and one of 18 colorectal carcinomas (6%) had easily detected RNA signals. (2) Immunohistochemical staining: all non‐malignant samples of gastric and colonic mucosa contained CgA‐positive neuroendocrine (NE) cells. Two of 17 (12%) gastric adenocarcinomas, and 3 of 18 (17%) of colorectal adenocarcinomas contained CgA‐positive tumor cells. Interestingly, the positive cases detected by immunohistochemistry included both cases detected by Northern blotting. Of the 5 cases detected by immunohistochemistry, 2 gastric cancers and I rectal carcinoma contained many diffusely scattered positive cells, occurring singly or in small clusters, while 2 colorectal carcinomas contained only occasional single CgA‐positive tumor cells. In one of the positive gastric cases, a well‐differentiated adenocarcinoma arising in a tubular adenoma, both the adenomatous and the carcinomatous elements contained positively staining cells. Our specific assays for CgA indicate that (I) a NE cell component, either diffusely scattered or occasional, occurs in about 15% of gastric and colorectal tumors; (2) there is no correlation between the presence of NE cells and degree of tumor differentiation; and (3) because only a minority of the tumor cells in positive cases stain for CgA, immunohistochemistry is a more sensitive method than Northern blotting.

Original languageEnglish (US)
Pages (from-to)189-194
Number of pages6
JournalInternational Journal of Cancer
Issue number2
StatePublished - May 8 1992
Externally publishedYes

ASJC Scopus subject areas

  • Oncology
  • Cancer Research


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