TY - GEN
T1 - Chondrogenic differentiation of human embryonic germ cell derived cells in hydrogels
AU - Varghese, Shyni
AU - Theprungsirikul, Paranduangji
AU - Ferran, Angela
AU - Hwang, Nathaniel
AU - Canver, Adam
AU - Elisseeff, Jennifer
PY - 2006
Y1 - 2006
N2 - Human embryonic germ (hEG) cells have the potential to self-renew over long periods of time and differentiate into various lineages. Cells derived from embryoid bodies of hEG cells express a broad spectrum of gene markers and have been induced towards cells of ectodermal and recently endo-dermal and mesenchymal lineages. LVEC cells express a number of surface marker proteins characteristic of mesenchymal stem cells (MSCs), indicating the potential of these cells to differentiate into mesenchymal tissues. Here we demonstrate the homogenous differentiation of LVEC cells into hyaline cartilage. Three dimensional tissue formation is achieved by encapsulating cells in synthetic hydrogels followed by incubation in chondrocyte-conditioned culture medium. Homogenous hyaline cartilage was produced, even after 63 population doublings (13 passages). The high proliferative capacity of these cells without teratoma formation, homogenous differentiation, and three-dimensional cartilage tissue formation suggests the significant potential of LVEC cells for cartilage tissue engineering applications.
AB - Human embryonic germ (hEG) cells have the potential to self-renew over long periods of time and differentiate into various lineages. Cells derived from embryoid bodies of hEG cells express a broad spectrum of gene markers and have been induced towards cells of ectodermal and recently endo-dermal and mesenchymal lineages. LVEC cells express a number of surface marker proteins characteristic of mesenchymal stem cells (MSCs), indicating the potential of these cells to differentiate into mesenchymal tissues. Here we demonstrate the homogenous differentiation of LVEC cells into hyaline cartilage. Three dimensional tissue formation is achieved by encapsulating cells in synthetic hydrogels followed by incubation in chondrocyte-conditioned culture medium. Homogenous hyaline cartilage was produced, even after 63 population doublings (13 passages). The high proliferative capacity of these cells without teratoma formation, homogenous differentiation, and three-dimensional cartilage tissue formation suggests the significant potential of LVEC cells for cartilage tissue engineering applications.
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U2 - 10.1109/IEMBS.2006.259710
DO - 10.1109/IEMBS.2006.259710
M3 - Conference contribution
C2 - 17946525
AN - SCOPUS:34047170998
SN - 1424400325
SN - 9781424400324
T3 - Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
SP - 2643
EP - 2646
BT - 28th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS'06
T2 - 28th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS'06
Y2 - 30 August 2006 through 3 September 2006
ER -