Choline: High-affinity uptake by rat brain synaptosomes

Henry I. Yamamura; Solomon H. Snyder

doi:10.1126/science.178.4061.626

Choline: High-affinity uptake by rat brain synaptosomes

Henry I. Yamamura, Solomon H. Snyder

School of Medicine

Research output: Contribution to journal › Article › peer-review

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Abstract

Synaptosomes from rat brain accumulate choline by two kinetically distinct processes, a high-affinity uptake system [Michaelis constant (K_m) = 1 X 10^-6M], and a low-affinity system (K_m = 9 X 10 ^-5M). The high-affinity uptake system requires sodium, and is associated with considerable formation of acetylcholine. The low-affinity uptake system is less dependent on sodium, and does not appear to be associated with a marked degree of acetylcholine formation. The high-affinity choline uptake appears to represent selective choline accumulation by cholinergic neurons.

Original language	English (US)
Pages (from-to)	626-628
Number of pages	3
Journal	Science
Volume	178
Issue number	4061
DOIs	https://doi.org/10.1126/science.178.4061.626
State	Published - Jan 1 1972

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title = "Choline: High-affinity uptake by rat brain synaptosomes",

abstract = "Synaptosomes from rat brain accumulate choline by two kinetically distinct processes, a high-affinity uptake system [Michaelis constant (Km) = 1 X 10-6M], and a low-affinity system (Km = 9 X 10 -5M). The high-affinity uptake system requires sodium, and is associated with considerable formation of acetylcholine. The low-affinity uptake system is less dependent on sodium, and does not appear to be associated with a marked degree of acetylcholine formation. The high-affinity choline uptake appears to represent selective choline accumulation by cholinergic neurons.",

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year = "1972",

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AB - Synaptosomes from rat brain accumulate choline by two kinetically distinct processes, a high-affinity uptake system [Michaelis constant (Km) = 1 X 10-6M], and a low-affinity system (Km = 9 X 10 -5M). The high-affinity uptake system requires sodium, and is associated with considerable formation of acetylcholine. The low-affinity uptake system is less dependent on sodium, and does not appear to be associated with a marked degree of acetylcholine formation. The high-affinity choline uptake appears to represent selective choline accumulation by cholinergic neurons.

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Choline: High-affinity uptake by rat brain synaptosomes

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