Chip-based DNA separation in free solution by inertial hydrodynamic forces

Jen Kuei Wu, Sarah Friedrich, Kelvin J. Liu, Tza Huei Wang

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We report a microfluidic chip capable of carrying out separations of wide range DNA fragments in free solution by hydrodynamic separation. Hind III digested λ DNA fragments, ranging from 564 to 23,130 bp, were successfully separated in a 2 μm diameter channel by pressure-driven transport. Separations obtained in 15 minutes show the high performance of the device and the specific sizing resolution of small size DNA fragments with 2×105 plate number. To the authors' knowledge, this is the first report on DNA separation on a microfluidic chip without the use of sieving matrices or pulsed electric fields.

Original languageEnglish (US)
Title of host publication17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
PublisherChemical and Biological Microsystems Society
Pages578-580
Number of pages3
ISBN (Print)9781632666246
StatePublished - 2013
Event17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 - Freiburg, Germany
Duration: Oct 27 2013Oct 31 2013

Publication series

Name17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
Volume1

Conference

Conference17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
Country/TerritoryGermany
CityFreiburg
Period10/27/1310/31/13

Keywords

  • Free solution
  • Hydrodynamic flow
  • Wide range DNA fragments separation

ASJC Scopus subject areas

  • Bioengineering

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