Abstract
As our closest living relatives, non-human primates uniquely enable explorations of human health, disease, development, and evolution. Considerable effort has thus been devoted to generating induced pluripotent stem cells (iPSCs) from multiple non-human primate species. Here, we establish improved culture methods for chimpanzee (Pan troglodytes) and pig-tailed macaque (Macaca nemestrina) iPSCs. Such iPSCs spontaneously differentiate in conventional culture conditions, but can be readily propagated by inhibiting endogenous WNT signaling. As a unique functional test of these iPSCs, we injected them into the pre-implantation embryos of another non-human species, rhesus macaques (Macaca mulatta). Ectopic expression of gene BCL2 enhances the survival and proliferation of chimpanzee and pig-tailed macaque iPSCs within the pre-implantation embryo, although the identity and long-term contribution of the transplanted cells warrants further investigation. In summary, we disclose transcriptomic and proteomic data, cell lines, and cell culture resources that may be broadly enabling for non-human primate iPSCs research.
Original language | English (US) |
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Article number | 111264 |
Journal | Cell Reports |
Volume | 40 |
Issue number | 9 |
DOIs | |
State | Published - Aug 30 2022 |
Keywords
- CP: Developmental biology
- Nonhuman primates
- blastocyst, BCL2
- chimpanzee
- cross-species embryos
- iPSCs
- interspecies chimera
- pig-tailed macaque
ASJC Scopus subject areas
- General Biochemistry, Genetics and Molecular Biology