TY - JOUR
T1 - Childhood sarcomas
T2 - fine-needle aspiration cytopathology with an emphasis on the use of molecular studies
AU - Wangsiricharoen, Sintawat
AU - Fuller, Maren Y.
AU - Wakely, Paul E.
AU - Ali, Syed Z.
N1 - Publisher Copyright:
© 2022 American Society of Cytopathology
PY - 2023/1/1
Y1 - 2023/1/1
N2 - Introduction: In children and adolescents, most sarcoma subtypes have a simple karyotype with a single genetic alteration; cytologic findings combined with ancillary testing can lead to a specific diagnosis. The goal of this study was to review the use of fine-needle aspiration in conjunction with immunohistochemistry and molecular studies as a part of an integrated, multidisciplinary diagnostic workup for bone and soft tissue sarcomas in this population. Materials and methods: We searched for cases aged ≤18 years old with a malignant bone or soft tissue tumor that had corresponding cytology specimens from 3 institutions. Clinical data, cytologic findings and diagnoses, histologic diagnoses, and ancillary testing were documented. Results: Of 99 cases, 55% were male with a mean age of 12 years. Ninety-four cases (95%) had a specific histologic diagnosis, and 84 cases (85%) were primary neoplasms. Ninety-four cases (95%) had a malignant cytologic diagnosis, and 71 cases (72%) had a specific cytologic diagnosis concordant with the histologic diagnosis. Among primary tumors with a specific histologic diagnosis, a specific cytologic diagnosis was made in 63 cases (79%). After excluding osteosarcoma, 74% of the tumors (n = 50) had molecular studies. Specific genetic alterations supporting a definitive diagnosis were found in 42 cases (84%), the majority of which were demonstrated using Fluorescence In Situ Hybridization (n = 33, 79%). Conclusions: We found that fine-needle aspiration in conjunction with core needle biopsy, immunohistochemistry, and molecular studies allowed cytopathologists to accurately classify sarcomas in a pediatric age group.
AB - Introduction: In children and adolescents, most sarcoma subtypes have a simple karyotype with a single genetic alteration; cytologic findings combined with ancillary testing can lead to a specific diagnosis. The goal of this study was to review the use of fine-needle aspiration in conjunction with immunohistochemistry and molecular studies as a part of an integrated, multidisciplinary diagnostic workup for bone and soft tissue sarcomas in this population. Materials and methods: We searched for cases aged ≤18 years old with a malignant bone or soft tissue tumor that had corresponding cytology specimens from 3 institutions. Clinical data, cytologic findings and diagnoses, histologic diagnoses, and ancillary testing were documented. Results: Of 99 cases, 55% were male with a mean age of 12 years. Ninety-four cases (95%) had a specific histologic diagnosis, and 84 cases (85%) were primary neoplasms. Ninety-four cases (95%) had a malignant cytologic diagnosis, and 71 cases (72%) had a specific cytologic diagnosis concordant with the histologic diagnosis. Among primary tumors with a specific histologic diagnosis, a specific cytologic diagnosis was made in 63 cases (79%). After excluding osteosarcoma, 74% of the tumors (n = 50) had molecular studies. Specific genetic alterations supporting a definitive diagnosis were found in 42 cases (84%), the majority of which were demonstrated using Fluorescence In Situ Hybridization (n = 33, 79%). Conclusions: We found that fine-needle aspiration in conjunction with core needle biopsy, immunohistochemistry, and molecular studies allowed cytopathologists to accurately classify sarcomas in a pediatric age group.
KW - Childhood sarcoma
KW - Cytopathology
KW - Fine-needle aspiration
KW - Molecular
KW - Pediatric sarcoma
KW - Sarcoma
UR - http://www.scopus.com/inward/record.url?scp=85140096361&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85140096361&partnerID=8YFLogxK
U2 - 10.1016/j.jasc.2022.08.003
DO - 10.1016/j.jasc.2022.08.003
M3 - Article
C2 - 36270912
AN - SCOPUS:85140096361
SN - 2213-2945
VL - 12
SP - 20
EP - 29
JO - Journal of the American Society of Cytopathology
JF - Journal of the American Society of Cytopathology
IS - 1
ER -