Chemical synthesis of the mouse mitochondrial genome

Daniel G. Gibson; Hamilton O. Smith; Clyde A. Hutchison; J. Craig Venter; Chuck Merryman

doi:10.1038/nmeth.1515

Chemical synthesis of the mouse mitochondrial genome

Daniel G. Gibson
, Hamilton O. Smith
, Clyde A. Hutchison
, J. Craig Venter
, Chuck Merryman

Research output: Contribution to journal › Article › peer-review

Abstract

We describe a one-step, isothermal assembly method for synthesizing DNA molecules from overlapping oligonucleotides. The method cycles between in vitro recombination and amplification until the desired length is reached. As a demonstration of its simplicity and robustness, we synthesized the entire 16.3-kilobase mouse mitochondrial genome from 600 overlapping 60-mers.

Original language	English (US)
Pages (from-to)	901-903
Number of pages	3
Journal	Nature Methods
Volume	7
Issue number	11
DOIs	https://doi.org/10.1038/nmeth.1515
State	Published - Nov 2010
Externally published	Yes

ASJC Scopus subject areas

Biotechnology
Biochemistry
Molecular Biology
Cell Biology

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10.1038/nmeth.1515

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abstract = "We describe a one-step, isothermal assembly method for synthesizing DNA molecules from overlapping oligonucleotides. The method cycles between in vitro recombination and amplification until the desired length is reached. As a demonstration of its simplicity and robustness, we synthesized the entire 16.3-kilobase mouse mitochondrial genome from 600 overlapping 60-mers.",

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AU - Gibson, Daniel G.

AU - Smith, Hamilton O.

AU - Hutchison, Clyde A.

AU - Venter, J. Craig

AU - Merryman, Chuck

PY - 2010/11

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N2 - We describe a one-step, isothermal assembly method for synthesizing DNA molecules from overlapping oligonucleotides. The method cycles between in vitro recombination and amplification until the desired length is reached. As a demonstration of its simplicity and robustness, we synthesized the entire 16.3-kilobase mouse mitochondrial genome from 600 overlapping 60-mers.

AB - We describe a one-step, isothermal assembly method for synthesizing DNA molecules from overlapping oligonucleotides. The method cycles between in vitro recombination and amplification until the desired length is reached. As a demonstration of its simplicity and robustness, we synthesized the entire 16.3-kilobase mouse mitochondrial genome from 600 overlapping 60-mers.

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UR - https://www.scopus.com/pages/publications/78049323797#tab=citedBy

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VL - 7

SP - 901

EP - 903

JO - Nature Methods

JF - Nature Methods

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Chemical synthesis of the mouse mitochondrial genome

Abstract

ASJC Scopus subject areas

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