Characterization of unrelated clinical Legionella pneumophila isolates in Catalonia by monoclonal subgrouping and sequence-based typing

Marian Garcia-Nuñez; Sara Quero; Maria Lluisa Pedro-Botet; Irene Barrabeig; Josep Avarez; Irene Campoy; Maria Rosa Sala; Noemí Parraga-Niño; Sofia Minguell; Joan Caylà; Lourdes Mateu; Miquel Sabria

doi:10.2217/fmb-2016-0013

Characterization of unrelated clinical Legionella pneumophila isolates in Catalonia by monoclonal subgrouping and sequence-based typing

Marian Garcia-Nuñez
, Sara Quero
, Maria Lluisa Pedro-Botet
, Irene Barrabeig
, Josep Avarez
, Irene Campoy
, Maria Rosa Sala
, Noemí Parraga-Niño
, Sofia Minguell
, Joan Caylà
, Lourdes Mateu
, Miquel Sabria

Bloomberg School of Public Health

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Aim: To characterize the genetic diversity of unrelated Legionella pneumophila clinical isolates in Catalonia and compare with other European regions. Methods: 95 unrelated isolates were analyzed using monoclonal antibodies and sequence-based typing, 1989-2013. Results: The isolates showed a high diversity (IOD 0.964) with a predominance of some profiles (ST37-Phialdelphia, ST23-Philadelphia and ST1-OLDA). All regions had predominant sequence types (STs) that differed between regions, and only 3% of STs were shared between the three regions. Conclusion: L. pneumophila clinical isolates from Catalonia presented a high diversity and can be used in epidemiological surveillance studies. The heterogeneous predominance of STs between European regions suggested a relationship between geographical distribution and virulence of some STs.

Original language	English (US)
Pages (from-to)	865-875
Number of pages	11
Journal	Future microbiology
Volume	11
Issue number	7
DOIs	https://doi.org/10.2217/fmb-2016-0013
State	Published - Jul 2016

Keywords

Legionella
clinical isolates
monoclonal antibody
sequence-based typing

ASJC Scopus subject areas

Microbiology
Microbiology (medical)

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10.2217/fmb-2016-0013

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Garcia-Nuñez, M., Quero, S., Pedro-Botet, M. L., Barrabeig, I., Avarez, J., Campoy, I., Sala, M. R., Parraga-Niño, N., Minguell, S., Caylà, J., Mateu, L., & Sabria, M. (2016). Characterization of unrelated clinical Legionella pneumophila isolates in Catalonia by monoclonal subgrouping and sequence-based typing. Future microbiology, 11(7), 865-875. https://doi.org/10.2217/fmb-2016-0013

Garcia-Nuñez, M, Quero, S, Pedro-Botet, ML, Barrabeig, I, Avarez, J, Campoy, I, Sala, MR, Parraga-Niño, N, Minguell, S, Caylà, J, Mateu, L & Sabria, M 2016, 'Characterization of unrelated clinical Legionella pneumophila isolates in Catalonia by monoclonal subgrouping and sequence-based typing', Future microbiology, vol. 11, no. 7, pp. 865-875. https://doi.org/10.2217/fmb-2016-0013

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title = "Characterization of unrelated clinical Legionella pneumophila isolates in Catalonia by monoclonal subgrouping and sequence-based typing",

abstract = "Aim: To characterize the genetic diversity of unrelated Legionella pneumophila clinical isolates in Catalonia and compare with other European regions. Methods: 95 unrelated isolates were analyzed using monoclonal antibodies and sequence-based typing, 1989-2013. Results: The isolates showed a high diversity (IOD 0.964) with a predominance of some profiles (ST37-Phialdelphia, ST23-Philadelphia and ST1-OLDA). All regions had predominant sequence types (STs) that differed between regions, and only 3\% of STs were shared between the three regions. Conclusion: L. pneumophila clinical isolates from Catalonia presented a high diversity and can be used in epidemiological surveillance studies. The heterogeneous predominance of STs between European regions suggested a relationship between geographical distribution and virulence of some STs.",

keywords = "Legionella, clinical isolates, monoclonal antibody, sequence-based typing",

author = "Marian Garcia-Nu{\~n}ez and Sara Quero and Pedro-Botet, \{Maria Lluisa\} and Irene Barrabeig and Josep Avarez and Irene Campoy and Sala, \{Maria Rosa\} and Noem{\'i} Parraga-Ni{\~n}o and Sofia Minguell and Joan Cayl{\`a} and Lourdes Mateu and Miquel Sabria",

note = "Funding Information: The present study was funded by the project PI10/0191, integrated in the National R + D + I and funded by the ISCIII-General Evalutation Branch and the European Regional Development Fund (FEDER) by CIBER de Enfermedades Respiratorias (CIBERES). CIBERES is an initiative of Instituo de Salud Carlos III. S Quero was funded by CIBERES and FI-AGAUR (FI-AGAUR 2012). The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript. Publisher Copyright: {\textcopyright} 2016 Future Medicine Ltd.",

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doi = "10.2217/fmb-2016-0013",

language = "English (US)",

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T1 - Characterization of unrelated clinical Legionella pneumophila isolates in Catalonia by monoclonal subgrouping and sequence-based typing

AU - Garcia-Nuñez, Marian

AU - Quero, Sara

AU - Pedro-Botet, Maria Lluisa

AU - Barrabeig, Irene

AU - Avarez, Josep

AU - Campoy, Irene

AU - Sala, Maria Rosa

AU - Parraga-Niño, Noemí

AU - Minguell, Sofia

AU - Caylà, Joan

AU - Mateu, Lourdes

AU - Sabria, Miquel

N1 - Funding Information: The present study was funded by the project PI10/0191, integrated in the National R + D + I and funded by the ISCIII-General Evalutation Branch and the European Regional Development Fund (FEDER) by CIBER de Enfermedades Respiratorias (CIBERES). CIBERES is an initiative of Instituo de Salud Carlos III. S Quero was funded by CIBERES and FI-AGAUR (FI-AGAUR 2012). The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript. Publisher Copyright: © 2016 Future Medicine Ltd.

PY - 2016/7

Y1 - 2016/7

N2 - Aim: To characterize the genetic diversity of unrelated Legionella pneumophila clinical isolates in Catalonia and compare with other European regions. Methods: 95 unrelated isolates were analyzed using monoclonal antibodies and sequence-based typing, 1989-2013. Results: The isolates showed a high diversity (IOD 0.964) with a predominance of some profiles (ST37-Phialdelphia, ST23-Philadelphia and ST1-OLDA). All regions had predominant sequence types (STs) that differed between regions, and only 3% of STs were shared between the three regions. Conclusion: L. pneumophila clinical isolates from Catalonia presented a high diversity and can be used in epidemiological surveillance studies. The heterogeneous predominance of STs between European regions suggested a relationship between geographical distribution and virulence of some STs.

AB - Aim: To characterize the genetic diversity of unrelated Legionella pneumophila clinical isolates in Catalonia and compare with other European regions. Methods: 95 unrelated isolates were analyzed using monoclonal antibodies and sequence-based typing, 1989-2013. Results: The isolates showed a high diversity (IOD 0.964) with a predominance of some profiles (ST37-Phialdelphia, ST23-Philadelphia and ST1-OLDA). All regions had predominant sequence types (STs) that differed between regions, and only 3% of STs were shared between the three regions. Conclusion: L. pneumophila clinical isolates from Catalonia presented a high diversity and can be used in epidemiological surveillance studies. The heterogeneous predominance of STs between European regions suggested a relationship between geographical distribution and virulence of some STs.

KW - Legionella

KW - clinical isolates

KW - monoclonal antibody

KW - sequence-based typing

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JO - Future microbiology

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Characterization of unrelated clinical Legionella pneumophila isolates in Catalonia by monoclonal subgrouping and sequence-based typing

Abstract

Keywords

ASJC Scopus subject areas

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