Characterization of the insulin and transferrin receptors of two cancer cell lines

Yue Hua Tang; Xin Zhang; Shu Li Xia; Nai He Jing; You Min Feng

Characterization of the insulin and transferrin receptors of two cancer cell lines

Yue Hua Tang, Xin Zhang, Shu Li Xia, Nai He Jing, You Min Feng

Research output: Contribution to journal › Article › peer-review

Abstract

The insulin and transferrin receptors of the PA-1 and GR2H6 cell lines were characterized respectively. The optimum pH for the binding of PA-1 cell is 7.0 with insulin and with transforrin is 7.5∼8.0. Seatchard plot and Hill plot analyses show respectively the receptor number of 2.97 × 10⁶/cell and 6.21 × 10⁷/cell for insulin receptors of PA-1 and GR2H6 with kD of 4.16 × 10^-7M and 7.57 × 10^-7M, Hill coefficient of 0.78 and 0.82, showing a negative cooperativity; transferrin receptors of PA-1 and GR2H6 were found to have respectively receptor number of 1.25 × 10⁵/cell and 1.40 × 10⁵/cell, with kD of 1.38 × 10^-9M and 1.66 × 10^-8M. The Hill coefficient was 1.04 and 0.98, almost no different from 1, so transferrin receptors showed no cooperativity.

Original language	English (US)
Number of pages	1
Journal	Acta Biochimica et Biophysica Sinica
Volume	28
Issue number	4
State	Published - Dec 1 1996
Externally published	Yes

Keywords

Human ovarian teratocarcinoma-derived cell line PA-1
Insulin receptor
Mouse breast cancer cell line GR2H6
Transferrin receptor

ASJC Scopus subject areas

Biophysics
Biochemistry

Cite this

@article{73dc058fbb2b4473bcfe4cb886cebe00,

title = "Characterization of the insulin and transferrin receptors of two cancer cell lines",

abstract = "The insulin and transferrin receptors of the PA-1 and GR2H6 cell lines were characterized respectively. The optimum pH for the binding of PA-1 cell is 7.0 with insulin and with transforrin is 7.5∼8.0. Seatchard plot and Hill plot analyses show respectively the receptor number of 2.97 × 106/cell and 6.21 × 107/cell for insulin receptors of PA-1 and GR2H6 with kD of 4.16 × 10-7M and 7.57 × 10-7M, Hill coefficient of 0.78 and 0.82, showing a negative cooperativity; transferrin receptors of PA-1 and GR2H6 were found to have respectively receptor number of 1.25 × 105/cell and 1.40 × 105/cell, with kD of 1.38 × 10-9M and 1.66 × 10-8M. The Hill coefficient was 1.04 and 0.98, almost no different from 1, so transferrin receptors showed no cooperativity.",

keywords = "Human ovarian teratocarcinoma-derived cell line PA-1, Insulin receptor, Mouse breast cancer cell line GR2H6, Transferrin receptor",

author = "Tang, {Yue Hua} and Xin Zhang and Xia, {Shu Li} and Jing, {Nai He} and Feng, {You Min}",

year = "1996",

month = dec,

day = "1",

language = "English (US)",

volume = "28",

journal = "Acta Biochimica et Biophysica Sinica",

issn = "0582-9879",

publisher = "Science Press",

number = "4",

}

TY - JOUR

T1 - Characterization of the insulin and transferrin receptors of two cancer cell lines

AU - Tang, Yue Hua

AU - Zhang, Xin

AU - Xia, Shu Li

AU - Jing, Nai He

AU - Feng, You Min

PY - 1996/12/1

Y1 - 1996/12/1

N2 - The insulin and transferrin receptors of the PA-1 and GR2H6 cell lines were characterized respectively. The optimum pH for the binding of PA-1 cell is 7.0 with insulin and with transforrin is 7.5∼8.0. Seatchard plot and Hill plot analyses show respectively the receptor number of 2.97 × 106/cell and 6.21 × 107/cell for insulin receptors of PA-1 and GR2H6 with kD of 4.16 × 10-7M and 7.57 × 10-7M, Hill coefficient of 0.78 and 0.82, showing a negative cooperativity; transferrin receptors of PA-1 and GR2H6 were found to have respectively receptor number of 1.25 × 105/cell and 1.40 × 105/cell, with kD of 1.38 × 10-9M and 1.66 × 10-8M. The Hill coefficient was 1.04 and 0.98, almost no different from 1, so transferrin receptors showed no cooperativity.

AB - The insulin and transferrin receptors of the PA-1 and GR2H6 cell lines were characterized respectively. The optimum pH for the binding of PA-1 cell is 7.0 with insulin and with transforrin is 7.5∼8.0. Seatchard plot and Hill plot analyses show respectively the receptor number of 2.97 × 106/cell and 6.21 × 107/cell for insulin receptors of PA-1 and GR2H6 with kD of 4.16 × 10-7M and 7.57 × 10-7M, Hill coefficient of 0.78 and 0.82, showing a negative cooperativity; transferrin receptors of PA-1 and GR2H6 were found to have respectively receptor number of 1.25 × 105/cell and 1.40 × 105/cell, with kD of 1.38 × 10-9M and 1.66 × 10-8M. The Hill coefficient was 1.04 and 0.98, almost no different from 1, so transferrin receptors showed no cooperativity.

KW - Human ovarian teratocarcinoma-derived cell line PA-1

KW - Insulin receptor

KW - Mouse breast cancer cell line GR2H6

KW - Transferrin receptor

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M3 - Article

AN - SCOPUS:20444413464

SN - 0582-9879

VL - 28

JO - Acta Biochimica et Biophysica Sinica

JF - Acta Biochimica et Biophysica Sinica

IS - 4

ER -

Characterization of the insulin and transferrin receptors of two cancer cell lines

Abstract

Keywords

ASJC Scopus subject areas

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