Characterization of human papillomavirus type 45, a new type 18-related virus of the genital tract

Z. S. Naghashfar, N. B. Rosenshein, A. T. Lorincz, J. Buscema, K. V. Shah

Research output: Contribution to journalArticlepeer-review

24 Scopus citations


DNA of human papillomavirus (HPV) type 45, a new HPV type 18-related papillomavirus of the genital tract, was cloned from a recurrent cervical lesion displaying mild to moderate dysplasia with koilocytosis. HPV-45 DNA was identified in paraffin sections of biopsies of both the initial and recurrent lesions of the patient, taken 7 months apart. HPV-45 DNA hybridized efficiently to that of many different HPV types under low and moderate stringency conditins (T(m)-37°C to T(m)-25°C) but with only HPV-18 DNA under high stringency conditions (T(m)-17°C). HPV-45 DNA was distinguished from HPV-18 DNA by (i) differences in restriction enzyme digest patterns, (ii) lack of hybridization at T(m)-17°C between HPV-18 and some fragments of HPV-45, (iii) a value of 25% in liquid reassociation kinetics between HPV-18 and HPV-45 and (iv) differences in intensities of hybridization with selected tissue DNAs. The prevalence of HPV-45 infection in the genital tract was low. In tests of over 600 tissue DNAs from female genital tract lesions, HPV-45 sequences were detected in three additional tissues, one each of invasive cervical carcinoma, condyloma, and normal cervical epithelium. HPV-45 is a newly recognized papillomavirus which rarely infects the genital tract and is associated with lesions across a wide histological spectrum.

Original languageEnglish (US)
Pages (from-to)3073-3079
Number of pages7
JournalJournal of General Virology
Issue number12
StatePublished - 1987

ASJC Scopus subject areas

  • Virology


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