@article{7ca080f5da0d4d0887221baad90a39f9,
title = "Characterization of histone inheritance patterns in the Drosophila female germline",
abstract = "Stem cells have the unique ability to undergo asymmetric division which produces two daughter cells that are genetically identical, but commit to different cell fates. The loss of this balanced asymmetric outcome can lead to many diseases, including cancer and tissue dystrophy. Understanding this tightly regulated process is crucial in developing methods to treat these abnormalities. Here, we report that during a Drosophila female germline stem cell asymmetric division, the two daughter cells differentially inherit histones at key genes related to either maintaining the stem cell state or promoting differentiation, but not at constitutively active or silenced genes. We combine histone labeling with DNA Oligopaints to distinguish old versus new histones and visualize their inheritance patterns at a single-gene resolution in asymmetrically dividing cells in vivo. This strategy can be applied to other biological systems involving cell fate change during development or tissue homeostasis in multicellular organisms.",
keywords = "Drosophila, FISH, asymmetric cell division, epigenetics, histone",
author = "Kahney, {Elizabeth W.} and Zion, {Emily H.} and Lydia Sohn and Kayla Viets-Layng and Robert Johnston and Xin Chen",
note = "Funding Information: We thank Drs. M. Van Doren, J. Gall, D. Drummond-Barbosa, A. Spradling, and X.C. laboratory members for insightful suggestions. We also thank T. Wu (Harvard University) and E. Joyce (University of Pennsylvania) and their laboratory members for thoughtful discussions and reagents. We thank Johns Hopkins Integrated Imaging Center for imaging. This work was supported by F31GM122339 (E.W.K.), F31DK122702 (E.H.Z.), F31EY026786 (K.V.L.), R01EY025598 (R.J.), R01GM112008, R35GM127075, and the Howard Hughes Medical Institute (Grant# 55108512) (X.C.). Funding Information: We thank Drs. M. Van Doren, J. Gall, D. Drummond‐Barbosa, A. Spradling, and X.C. laboratory members for insightful suggestions. We also thank T. Wu (Harvard University) and E. Joyce (University of Pennsylvania) and their laboratory members for thoughtful discussions and reagents. We thank Johns Hopkins Integrated Imaging Center for imaging. This work was supported by F31GM122339 (E.W.K.), F31DK122702 (E.H.Z.), F31EY026786 (K.V.L.), R01EY025598 (R.J.), R01GM112008, R35GM127075, and the Howard Hughes Medical Institute (Grant# 55108512) (X.C.). Publisher Copyright: {\textcopyright} 2021 The Authors. Published under the terms of the CC BY NC ND 4.0 license",
year = "2021",
month = jul,
day = "5",
doi = "10.15252/embr.202051530",
language = "English (US)",
volume = "22",
journal = "EMBO Reports",
issn = "1469-221X",
publisher = "Springer Science and Business Media Deutschland GmbH",
number = "7",
}