Characterization of "heavy" and "light" SV40-like particles from a patient with PML

Malcolm A. Martin; Lawrence D. Gelb; Claude Garon; Kenneth K. Takemoto; Teresa N.H. Lee; George H. Sack; Daniel Nathans

doi:10.1016/0042-6822(74)90214-1

Characterization of "heavy" and "light" SV40-like particles from a patient with PML

Malcolm A. Martin, Lawrence D. Gelb, Claude Garon, Kenneth K. Takemoto, Teresa N.H. Lee, George H. Sack, Daniel Nathans

School of Medicine

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

Two discrete classes of particles appear following the infection of primary African green monkey kidney cells with high multiplicities of an SV40-like isolate from a patient with progressive multifocal leukoencephalopathy (PML). The DNA purified from the nondefective "heavy" particles was very similar to standard SV40 DNA. "Light" viral DNA was circular, supercoiled, 80% the physical size of SV40, and did not contain the Eco_RI restriction endonuclease site. The kinetics of "light" viral DNA reassociation indicated a physical complexity equivalent to 40-50% of SV40 DNA; no host sequences were detected in "light" viral DNA. Digestion of "light" viral DNA with the Hemophilus influenzae restriction endonuclease resulted in 5 cleavage products none of which corresponded to the 11 fragments obtained with SV40 DNA.

Original language	English (US)
Pages (from-to)	179-189
Number of pages	11
Journal	Virology
Volume	59
Issue number	1
DOIs	https://doi.org/10.1016/0042-6822(74)90214-1
State	Published - May 1974

ASJC Scopus subject areas

Virology

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10.1016/0042-6822(74)90214-1

Cite this

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abstract = "Two discrete classes of particles appear following the infection of primary African green monkey kidney cells with high multiplicities of an SV40-like isolate from a patient with progressive multifocal leukoencephalopathy (PML). The DNA purified from the nondefective {"}heavy{"} particles was very similar to standard SV40 DNA. {"}Light{"} viral DNA was circular, supercoiled, 80% the physical size of SV40, and did not contain the EcoRI restriction endonuclease site. The kinetics of {"}light{"} viral DNA reassociation indicated a physical complexity equivalent to 40-50% of SV40 DNA; no host sequences were detected in {"}light{"} viral DNA. Digestion of {"}light{"} viral DNA with the Hemophilus influenzae restriction endonuclease resulted in 5 cleavage products none of which corresponded to the 11 fragments obtained with SV40 DNA.",

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AU - Martin, Malcolm A.

AU - Gelb, Lawrence D.

AU - Garon, Claude

AU - Takemoto, Kenneth K.

AU - Lee, Teresa N.H.

AU - Sack, George H.

AU - Nathans, Daniel

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AB - Two discrete classes of particles appear following the infection of primary African green monkey kidney cells with high multiplicities of an SV40-like isolate from a patient with progressive multifocal leukoencephalopathy (PML). The DNA purified from the nondefective "heavy" particles was very similar to standard SV40 DNA. "Light" viral DNA was circular, supercoiled, 80% the physical size of SV40, and did not contain the EcoRI restriction endonuclease site. The kinetics of "light" viral DNA reassociation indicated a physical complexity equivalent to 40-50% of SV40 DNA; no host sequences were detected in "light" viral DNA. Digestion of "light" viral DNA with the Hemophilus influenzae restriction endonuclease resulted in 5 cleavage products none of which corresponded to the 11 fragments obtained with SV40 DNA.

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Characterization of "heavy" and "light" SV40-like particles from a patient with PML

Abstract

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