Characterization of a Novel Transcript of Retroviral Origin Expressed in Rat Heart and Liver

Xavier J. Martin; Ketty Schwartz; Kenneth R. Boheler

doi:10.1016/S0022-2828(08)80053-3

Characterization of a Novel Transcript of Retroviral Origin Expressed in Rat Heart and Liver

Xavier J. Martin
, Ketty Schwartz
, Kenneth R. Boheler

Research output: Contribution to journal › Article › peer-review

Abstract

X. J. Martin, K. Schwartz and K. R. Boheler. Chracterization of a Novel Transcript of Retroviral Origin Expressed in Rat Heart and Liver. Journal of Molecular and Cellular Cardiology (1995) 27, 589-597. Eleven cDNA clones of retroviral origin (SORO) have been isolated and characterized from a rat cardiac cDNA library that are highly homologous to the RAL-element family of rat retroviral endogenous sequences. SORO-1, the largest of the clones isolated at approximately 3.5 kb, contains a characteristic long terminal repeat (LTR) that is unique to previously identified retroposons. Its LTR contains elements homologous to the CAAT box, a TATA box, a core enhancer sequence, and potential binding sites for GATA. SORO-1 hybridizes to a mRNA of approximately 7 kb, that is present both in rat heart and liver. This transcript was not, however, detected in other tissues (fast and slow skeletal muscles, brain, kidney, testis, lung, intestine, spleen)or from any other species (man, monkey mouse, dog, rabbit, chicken, cow, yeast) examined. During postnatal cardiac development, the expression of the transcript appears to be down-regulated and is present at levels 1-fold greater in neonates than in adults. The function of this retroposon that is expressed in rat heart and liver has not yet been determined, but the sequence anlysis of its LTR and its expression pattern suggest that it may be regulated by specific trans-acting factors that are present in both rat heart and liver.

Original language	English (US)
Pages (from-to)	589-597
Number of pages	9
Journal	Journal of Molecular and Cellular Cardiology
Volume	27
Issue number	1
DOIs	https://doi.org/10.1016/S0022-2828(08)80053-3
State	Published - Jan 1995
Externally published	Yes

Keywords

Endogenous retroviral sequence
Post-natal development
Tissue-specific regulation

ASJC Scopus subject areas

Molecular Biology
Cardiology and Cardiovascular Medicine

Access to Document

10.1016/S0022-2828(08)80053-3

Cite this

@article{affc80ab6edd4afaacf39c81301d2d92,

title = "Characterization of a Novel Transcript of Retroviral Origin Expressed in Rat Heart and Liver",

abstract = "X. J. Martin, K. Schwartz and K. R. Boheler. Chracterization of a Novel Transcript of Retroviral Origin Expressed in Rat Heart and Liver. Journal of Molecular and Cellular Cardiology (1995) 27, 589-597. Eleven cDNA clones of retroviral origin (SORO) have been isolated and characterized from a rat cardiac cDNA library that are highly homologous to the RAL-element family of rat retroviral endogenous sequences. SORO-1, the largest of the clones isolated at approximately 3.5 kb, contains a characteristic long terminal repeat (LTR) that is unique to previously identified retroposons. Its LTR contains elements homologous to the CAAT box, a TATA box, a core enhancer sequence, and potential binding sites for GATA. SORO-1 hybridizes to a mRNA of approximately 7 kb, that is present both in rat heart and liver. This transcript was not, however, detected in other tissues (fast and slow skeletal muscles, brain, kidney, testis, lung, intestine, spleen)or from any other species (man, monkey mouse, dog, rabbit, chicken, cow, yeast) examined. During postnatal cardiac development, the expression of the transcript appears to be down-regulated and is present at levels 1-fold greater in neonates than in adults. The function of this retroposon that is expressed in rat heart and liver has not yet been determined, but the sequence anlysis of its LTR and its expression pattern suggest that it may be regulated by specific trans-acting factors that are present in both rat heart and liver.",

keywords = "Endogenous retroviral sequence, Post-natal development, Tissue-specific regulation",

author = "Martin, \{Xavier J.\} and Ketty Schwartz and Boheler, \{Kenneth R.\}",

note = "Funding Information: We thank 1)rs Michael Wa\textasciitilde{}kerl and Henk ter Keurs Ibr constructive discussions and we would especially like to thank Dr tiered l:arza lbr the gift of lhe PCR-amplified DNA fragme\textasciitilde{}t, bdpfnl discussion and sequence analysis and search in the database. This work was supported by INSERM, E.E.C. (No BMItlCT 921l 71) and the Association Francaise centre les Myopathies. XM was supported by fellowships from the Association Franc'aise centre les Myopathies and the Ligue Nationale centre/e Cancer.",

year = "1995",

month = jan,

doi = "10.1016/S0022-2828(08)80053-3",

language = "English (US)",

volume = "27",

pages = "589--597",

journal = "Journal of Molecular and Cellular Cardiology",

issn = "0022-2828",

publisher = "Academic Press",

number = "1",

}

TY - JOUR

T1 - Characterization of a Novel Transcript of Retroviral Origin Expressed in Rat Heart and Liver

AU - Martin, Xavier J.

AU - Schwartz, Ketty

AU - Boheler, Kenneth R.

N1 - Funding Information: We thank 1)rs Michael Wa~kerl and Henk ter Keurs Ibr constructive discussions and we would especially like to thank Dr tiered l:arza lbr the gift of lhe PCR-amplified DNA fragme~t, bdpfnl discussion and sequence analysis and search in the database. This work was supported by INSERM, E.E.C. (No BMItlCT 921l 71) and the Association Francaise centre les Myopathies. XM was supported by fellowships from the Association Franc'aise centre les Myopathies and the Ligue Nationale centre/e Cancer.

PY - 1995/1

Y1 - 1995/1

N2 - X. J. Martin, K. Schwartz and K. R. Boheler. Chracterization of a Novel Transcript of Retroviral Origin Expressed in Rat Heart and Liver. Journal of Molecular and Cellular Cardiology (1995) 27, 589-597. Eleven cDNA clones of retroviral origin (SORO) have been isolated and characterized from a rat cardiac cDNA library that are highly homologous to the RAL-element family of rat retroviral endogenous sequences. SORO-1, the largest of the clones isolated at approximately 3.5 kb, contains a characteristic long terminal repeat (LTR) that is unique to previously identified retroposons. Its LTR contains elements homologous to the CAAT box, a TATA box, a core enhancer sequence, and potential binding sites for GATA. SORO-1 hybridizes to a mRNA of approximately 7 kb, that is present both in rat heart and liver. This transcript was not, however, detected in other tissues (fast and slow skeletal muscles, brain, kidney, testis, lung, intestine, spleen)or from any other species (man, monkey mouse, dog, rabbit, chicken, cow, yeast) examined. During postnatal cardiac development, the expression of the transcript appears to be down-regulated and is present at levels 1-fold greater in neonates than in adults. The function of this retroposon that is expressed in rat heart and liver has not yet been determined, but the sequence anlysis of its LTR and its expression pattern suggest that it may be regulated by specific trans-acting factors that are present in both rat heart and liver.

AB - X. J. Martin, K. Schwartz and K. R. Boheler. Chracterization of a Novel Transcript of Retroviral Origin Expressed in Rat Heart and Liver. Journal of Molecular and Cellular Cardiology (1995) 27, 589-597. Eleven cDNA clones of retroviral origin (SORO) have been isolated and characterized from a rat cardiac cDNA library that are highly homologous to the RAL-element family of rat retroviral endogenous sequences. SORO-1, the largest of the clones isolated at approximately 3.5 kb, contains a characteristic long terminal repeat (LTR) that is unique to previously identified retroposons. Its LTR contains elements homologous to the CAAT box, a TATA box, a core enhancer sequence, and potential binding sites for GATA. SORO-1 hybridizes to a mRNA of approximately 7 kb, that is present both in rat heart and liver. This transcript was not, however, detected in other tissues (fast and slow skeletal muscles, brain, kidney, testis, lung, intestine, spleen)or from any other species (man, monkey mouse, dog, rabbit, chicken, cow, yeast) examined. During postnatal cardiac development, the expression of the transcript appears to be down-regulated and is present at levels 1-fold greater in neonates than in adults. The function of this retroposon that is expressed in rat heart and liver has not yet been determined, but the sequence anlysis of its LTR and its expression pattern suggest that it may be regulated by specific trans-acting factors that are present in both rat heart and liver.

KW - Endogenous retroviral sequence

KW - Post-natal development

KW - Tissue-specific regulation

UR - https://www.scopus.com/pages/publications/0028958459

UR - https://www.scopus.com/pages/publications/0028958459#tab=citedBy

U2 - 10.1016/S0022-2828(08)80053-3

DO - 10.1016/S0022-2828(08)80053-3

M3 - Article

C2 - 7760379

AN - SCOPUS:0028958459

SN - 0022-2828

VL - 27

SP - 589

EP - 597

JO - Journal of Molecular and Cellular Cardiology

JF - Journal of Molecular and Cellular Cardiology

IS - 1

ER -

Characterization of a Novel Transcript of Retroviral Origin Expressed in Rat Heart and Liver

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this