Characterization of a novel PMA-inducible pathway of interleukin-13 gene expression in T cells

Judith C. Keen, Antonella Cianferoni, Giovanni Florio, Jia Guo, Rongbing Chen, Jessica Roman, Marsha Wills-Karp, Vincenzo Casolaro, Steve N. Georas

Research output: Contribution to journalArticlepeer-review

13 Scopus citations


Although interleukin 13 (IL-13) is an important mediator of asthma and allergic diseases, the molecular mechanisms regulating IL-13 gene expression are not well understood. This study was designed to define the molecular mechanisms governing IL-13 gene expression in T cells. IL-13 expression was examined in human peripheral blood T cells and in the EL-4 T-cell line by enzyme-linked immunosorbent assay and reverse-transcription polymerase chain reaction. An IL-13 promoter deletion analysis was performed using luciferase-based reporter plasmids transiently transfected into EL-4 cells by electroporation. DNA binding factors were investigated using electrophoretic mobility shift assays. In contrast to IL-4 expression, which required concomitant activation of calcium- and protein kinase C- (PKC-) dependent signalling pathways, PKC activation alone was sufficient for IL-13 protein secretion in mitogen-primed (but not resting) peripheral blood T cells, and for IL-13 mRNA expression and promoter activity in EL-4 T cells. Promoter deletion analysis localized a phorbol 12-myristate 13-acetate (PMA) -sensitive element to a proximal promoter region between -109 and -79 base pairs q2upstream from the IL-13 transcription start site. This promoter region supported the binding of both constitutive and PMA-inducible nuclear factors in gel shift assays.

Original languageEnglish (US)
Pages (from-to)29-37
Number of pages9
Issue number1
StatePublished - Jan 2006
Externally publishedYes


  • Interleukin 13
  • Protein kinase C
  • T lymphocyte
  • Transcriptional regulation

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology


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