Abstract
We examined the function of a highly conserved Histidine rich sequence of amino acids found in the carboxyl-terminal of the Na+/H+ exchanger (NHE1). A fusion protein containing the sequence HYGHHH (540-545) and the balance of the carboxyl terminal of the protein did not bind calcium but bound to an immobilized metal affinity column and could be used to partially purify the exchanger protein. Mutation of the sequence to either HYGAAA or HYGRRR did not affect activity of the intact protein. Mutation to HHHHHH did not affect proton activation of the Na+/H+ exchanger or localization but caused a decreased maximal velocity suggesting that this conserved sequence is important in maximal activity of the Na+/H+ exchanger.
Original language | English (US) |
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Pages (from-to) | 185-197 |
Number of pages | 13 |
Journal | Bioscience Reports |
Volume | 20 |
Issue number | 3 |
DOIs | |
State | Published - 2000 |
Externally published | Yes |
Keywords
- Histidine residues
- Na/H exchanger
- Proton sensing
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology