TY - JOUR
T1 - Characterization and differentiation of CD51+ Stem Leydig cells in adult mouse testes
AU - Chen, Panpan
AU - Guan, Xiaoju
AU - Zhao, Xingxing
AU - Chen, Fenfen
AU - Yang, Jianying
AU - Wang, Yiyan
AU - Hu, Yue
AU - Lian, Qingquan
AU - Chen, Haolin
N1 - Funding Information:
This work is supported by grants from Chinese central or local governments, including Natural Science Foundation of Zhejiang Province grant LY17H040012 (HC), Research Fund for Lin He’s Academician Workstation of New Medicine and Clinical Translation 17331101 (HC), Zhejiang Medical and Health Science and Technology Project 2019318176 (XG), and an open grant from Zhejiang Provincial Top Discipline of Clinical Medicine (HC and QL). These sources did not influence the study design, the collection, analysis or interpretation of data, or the writing of the paper.
Publisher Copyright:
© 2019 Elsevier B.V.
PY - 2019/8/1
Y1 - 2019/8/1
N2 - It was reported previously that adult mouse stem Leydig cells (SLCs) express CD51 (integrin α-chain V). However, it is still unclear whether all CD51+ cells are SLCs. In the present study, we found that CD51+ cells can be classified into two sub-groups, a weakly-staining group (CD51+) and a strongly-staining group (CD51++). The CD51+ cells expressed common SLC marker genes, including Nestin, Pdgfra and Coup-tf2, while CD51++ cells did not express these genes. Instead, they expressed macrophage markers, such as F4/80, Cd115 and Tnfa. When these cells were induced to differentiate in vitro, the CD51+ cells, but not CD51++ cells, formed Leydig cells. Overall, our results showed that although SLCs expressed CD51, not all CD51-expressing cells are SLCs. The cells that expressed high levels of CD51 are actually macrophages.
AB - It was reported previously that adult mouse stem Leydig cells (SLCs) express CD51 (integrin α-chain V). However, it is still unclear whether all CD51+ cells are SLCs. In the present study, we found that CD51+ cells can be classified into two sub-groups, a weakly-staining group (CD51+) and a strongly-staining group (CD51++). The CD51+ cells expressed common SLC marker genes, including Nestin, Pdgfra and Coup-tf2, while CD51++ cells did not express these genes. Instead, they expressed macrophage markers, such as F4/80, Cd115 and Tnfa. When these cells were induced to differentiate in vitro, the CD51+ cells, but not CD51++ cells, formed Leydig cells. Overall, our results showed that although SLCs expressed CD51, not all CD51-expressing cells are SLCs. The cells that expressed high levels of CD51 are actually macrophages.
KW - CD51
KW - Macrophage
KW - Stem Leydig cells
KW - Testis
KW - Testosterone
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U2 - 10.1016/j.mce.2019.110449
DO - 10.1016/j.mce.2019.110449
M3 - Article
C2 - 31102608
AN - SCOPUS:85066628744
SN - 0303-7207
VL - 493
JO - Molecular and Cellular Endocrinology
JF - Molecular and Cellular Endocrinology
M1 - 110449
ER -