This chapter discusses local heating by optical tweezers. Because most optical tweezers have been custom-built, if not customized for particular applications, estimates of aqueous heating are likely to vary between apparatuses. The chapter presents a simple, direct method to estimate the local heating by optical tweezers that will be useful to all practitioners of the technology. A number of methods exist for monitoring the local temperature near the field of observation. Microthermocouples can be inserted near the field of view, but accidental irradiation of the thermocouple by the optical tweezers laser can cause temperature jumps more than 500°C, which vaporize the local media.
ASJC Scopus subject areas
- Cell Biology