Chaperone-mediated autophagy targets hypoxia-inducible factor-1β(HIF- 1β) for lysosomal degradation

Background: Regulation of hypoxia-inducible factor-1 (HIF-1) is focused on proteasomal degradation of the HIF-1β subunit. Results: Pharmacological and genetic approaches establish that HIF-1βbinds to effectors of chaperone-mediated autophagy (CMA) and is targeted for lysosomal degradation. Conclusion: CMA targets HIF-1βfor lysosomal degradation. Significance: Lysosomal degradation of HIF-1βrepresents a novel mechanism of HIF-1 regulation and a potential therapeutic target.