Abstract
PURPOSE: The organization of scleral collagen helps to determine the eye's biomechanical response to intraocular pressure (IOP), and may therefore be important in glaucoma. This study provides a quantitative assessment of changes in scleral collagen fibril organization in bead-induced murine experimental glaucoma.
METHODS: Wide-angle X-ray scattering was used to study the effect of bead-induced glaucoma on posterior scleral collagen organization in one eye of 12 CD1 mice, with untreated fellow eyes serving as controls. Three collagen parameters were measured: the local preferred fibril directions, the degree of collagen anisotropy, and the total fibrillar collagen content.
RESULTS: The mouse sclera featured a largely circumferential orientation of fibrillar collagen with respect to the optic nerve head canal. Localized alteration to fibril orientations was evident in the inferior peripapillary sclera of bead-treated eyes. Collagen anisotropy was significantly (P<0.05)
CONTROL:
CONTROL:
CONTROL: 47±3%). No significant differences in total collagen content were found between groups.
CONCLUSIONS: Spatial changes in collagen fibril anisotropy occur in the posterior sclera of mice with bead-induced chronic IOP elevation and axonal damage. These results support the idea that dynamic changes in scleral form and structure play a role in the development of experimental glaucoma in mice, and potentially in human glaucoma.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 6554-6564 |
| Number of pages | 11 |
| Journal | Investigative ophthalmology & visual science |
| Volume | 55 |
| Issue number | 10 |
| DOIs | |
| State | Published - 2014 |
| Externally published | Yes |
Keywords
- X-ray scattering
- collagen
- glaucoma
- mouse
- sclera
ASJC Scopus subject areas
- Ophthalmology
- Sensory Systems
- Cellular and Molecular Neuroscience