Abstract
Laurell ‘rocket’ immunoelectrophoresis has been applied to the quantitation of lens crystallins during development of hereditary cataract in the Philly mouse. A marked decrease in γ crystallin and smaller loss of β crystallin was found to accompany catarac-togenesis. Both β and γ crystallins were identified in the aqueous humor of the Philly mouse following cataract development. The Philly mouse lens was shown to lack one β crystallin antigen seen in the normal mouse by the rocket technique. This observation was correlated with altered composition of β crystallin polypeptides and oligomers. The rocket method was also applied to quantitation of human α, β, and γ crystallin.
Original language | English (US) |
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Pages (from-to) | 237-251 |
Number of pages | 15 |
Journal | Ophthalmic Research |
Volume | 13 |
Issue number | 5 |
DOIs | |
State | Published - 1981 |
Keywords
- Cataract
- Crystallin quantitation
- Degradation
- Insolubilization
- Leakage
- Lens crystallins
- Philly mouse
- Rocket immunoelectrophoresis
ASJC Scopus subject areas
- Ophthalmology
- Sensory Systems
- Cellular and Molecular Neuroscience