Cellular uptake of cadmium and zinc

Alice J. Corrigan; P. C. Huang

doi:10.1007/BF02990117

Cellular uptake of cadmium and zinc

Alice J. Corrigan, P. C. Huang

Bloomberg School of Public Health

Research output: Contribution to journal › Article › peer-review

26 Scopus citations

Abstract

CHO mutants, resistant to over 100-fold of a normally toxic level of extracellular cadmium have been used to examine the mutually antagonistic effect of Cd and Zn on their uptake. Cadmium uptake in these mutants is only 7-10% that of the parental cells. Zinc uptake in these mutants is equal to or greater than that in the wild-type cells. Results of kinetic studies on uptake indicated that the two metals interact by competitive inhibition. The K_m and K_i values for Cd and/or Zn were different in some of the mutants and indicate multiple carriers may be involved in the transport of these metals. The reduction in Cd uptake and concomitant increase in Zn uptake contribute to the increased Cd resistance in these mutants.

Original language	English (US)
Pages (from-to)	197-216
Number of pages	20
Journal	Biological Trace Element Research
Volume	3
Issue number	3
DOIs	https://doi.org/10.1007/BF02990117
State	Published - Sep 1 1981

Keywords

CHO cells, Cd and Zn uptake in
Cadmium, cellular uptake of
antagonism, of Cd and Zn in cellular uptake
metallothionein, in cellular uptake of Cd and Zn
transport, of Cd and Zn in cellular uptake
zinc, cellular uptake of

ASJC Scopus subject areas

Endocrinology, Diabetes and Metabolism
Biochemistry
Clinical Biochemistry
Biochemistry, medical
Inorganic Chemistry

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abstract = "CHO mutants, resistant to over 100-fold of a normally toxic level of extracellular cadmium have been used to examine the mutually antagonistic effect of Cd and Zn on their uptake. Cadmium uptake in these mutants is only 7-10% that of the parental cells. Zinc uptake in these mutants is equal to or greater than that in the wild-type cells. Results of kinetic studies on uptake indicated that the two metals interact by competitive inhibition. The Km and Ki values for Cd and/or Zn were different in some of the mutants and indicate multiple carriers may be involved in the transport of these metals. The reduction in Cd uptake and concomitant increase in Zn uptake contribute to the increased Cd resistance in these mutants.",

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Cellular uptake of cadmium and zinc

Abstract

Keywords

ASJC Scopus subject areas

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