Cellular uptake and intracellular fate of antisense oligonucleotides

Alain R. Thierry; Eric Vives; Jean Philippe Richard; Paul Prevot; Camille Martinand-Mari; Ian Robbins; Bernard Lebleu

Cellular uptake and intracellular fate of antisense oligonucleotides

Alain R. Thierry, Eric Vives, Jean Philippe Richard, Paul Prevot, Camille Martinand-Mari, Ian Robbins, Bernard Lebleu

Research output: Contribution to journal › Review article › peer-review

Abstract

Antisense oligonucleotides and short interfering RNAs are routinely used for gene function analysis and are being developed for clinical applications. The mechanism underlying internalization of free oligonucleotides into cells is poorly understood and inefficient in most cases. Antisense oligonucleotide delivery into ex vivo cells is routinely improved by the addition of cationic lipids. New chemical modifications and vectors allowing improved cellular delivery in vivo are being developed.

Original language	English (US)
Pages (from-to)	133-138
Number of pages	6
Journal	Current Opinion in Molecular Therapeutics
Volume	5
Issue number	2
State	Published - Apr 2003
Externally published	Yes

Keywords

Antisense oligonucleotides
Cellular uptake
Delivery vectors

ASJC Scopus subject areas

Molecular Medicine
Molecular Biology
Genetics
Pharmacology
Drug Discovery
Genetics(clinical)

Cite this

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title = "Cellular uptake and intracellular fate of antisense oligonucleotides",

abstract = "Antisense oligonucleotides and short interfering RNAs are routinely used for gene function analysis and are being developed for clinical applications. The mechanism underlying internalization of free oligonucleotides into cells is poorly understood and inefficient in most cases. Antisense oligonucleotide delivery into ex vivo cells is routinely improved by the addition of cationic lipids. New chemical modifications and vectors allowing improved cellular delivery in vivo are being developed.",

keywords = "Antisense oligonucleotides, Cellular uptake, Delivery vectors",

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year = "2003",

month = apr,

language = "English (US)",

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T1 - Cellular uptake and intracellular fate of antisense oligonucleotides

AU - Thierry, Alain R.

AU - Vives, Eric

AU - Richard, Jean Philippe

AU - Prevot, Paul

AU - Martinand-Mari, Camille

AU - Robbins, Ian

AU - Lebleu, Bernard

PY - 2003/4

Y1 - 2003/4

N2 - Antisense oligonucleotides and short interfering RNAs are routinely used for gene function analysis and are being developed for clinical applications. The mechanism underlying internalization of free oligonucleotides into cells is poorly understood and inefficient in most cases. Antisense oligonucleotide delivery into ex vivo cells is routinely improved by the addition of cationic lipids. New chemical modifications and vectors allowing improved cellular delivery in vivo are being developed.

AB - Antisense oligonucleotides and short interfering RNAs are routinely used for gene function analysis and are being developed for clinical applications. The mechanism underlying internalization of free oligonucleotides into cells is poorly understood and inefficient in most cases. Antisense oligonucleotide delivery into ex vivo cells is routinely improved by the addition of cationic lipids. New chemical modifications and vectors allowing improved cellular delivery in vivo are being developed.

KW - Antisense oligonucleotides

KW - Cellular uptake

KW - Delivery vectors

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M3 - Review article

C2 - 12772502

AN - SCOPUS:0037799721

SN - 1464-8431

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EP - 138

JO - Current Opinion in Molecular Therapeutics

JF - Current Opinion in Molecular Therapeutics

IS - 2

ER -

Cellular uptake and intracellular fate of antisense oligonucleotides

Abstract

Keywords

ASJC Scopus subject areas

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