Cell surface expression of a specific antigenic site on the catalytic subunit of (Na+ + K+)-ATPase

Allen C. Myers; Bruce S. Bochner; Gordon F. Tomaselli; Neal Fedarko; Sherry A. Hudson; Holly Rohde; Shau Ku Huang; Kai Y. Xu

doi:10.1006/bbrc.2002.6419

Cell surface expression of a specific antigenic site on the catalytic subunit of (Na⁺ + K⁺)-ATPase

Allen C. Myers, Bruce S. Bochner, Gordon F. Tomaselli, Neal Fedarko, Sherry A. Hudson, Holly Rohde, Shau Ku Huang, Kai Y. Xu

School of Medicine

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Structural localization of a peptide region, KRQPRNPKTDKLVNE, in the catalytic subunit of (Na⁺ + K⁺)-ATPase was investigated using a specific antibody directed against this peptide in cultured African green monkey kidney CV-1 cells. Immunofluorescence staining of frozen cell sections shows that an anti-KRQPRNPKTDKLVNE antibody (SSA95) interacts with its antigenic site and binds to the extracellular side of the cell membrane. Indirect immunofluorescence and flow cytometric analyses confirmed the presence of this epitope on intact cell surfaces. These results suggest that the KRQPRNPKTDKLVNE region of the (Na⁺ + K⁺)-ATPase is expressed on the cellular membrane surface.

Original language	English (US)
Pages (from-to)	111-115
Number of pages	5
Journal	Biochemical and Biophysical Research Communications
Volume	291
Issue number	1
DOIs	https://doi.org/10.1006/bbrc.2002.6419
State	Published - 2002

Keywords

(Na + K)-ATPase
Flow cytometry
Immunofluorescence staining
Positive inotropy
Site-specific antibody

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

Access to Document

10.1006/bbrc.2002.6419

Cite this

@article{a330e646784440b1aabfa9d09bd89297,

title = "Cell surface expression of a specific antigenic site on the catalytic subunit of (Na+ + K+)-ATPase",

abstract = "Structural localization of a peptide region, KRQPRNPKTDKLVNE, in the catalytic subunit of (Na+ + K+)-ATPase was investigated using a specific antibody directed against this peptide in cultured African green monkey kidney CV-1 cells. Immunofluorescence staining of frozen cell sections shows that an anti-KRQPRNPKTDKLVNE antibody (SSA95) interacts with its antigenic site and binds to the extracellular side of the cell membrane. Indirect immunofluorescence and flow cytometric analyses confirmed the presence of this epitope on intact cell surfaces. These results suggest that the KRQPRNPKTDKLVNE region of the (Na+ + K+)-ATPase is expressed on the cellular membrane surface.",

keywords = "(Na + K)-ATPase, Flow cytometry, Immunofluorescence staining, Positive inotropy, Site-specific antibody",

author = "Myers, {Allen C.} and Bochner, {Bruce S.} and Tomaselli, {Gordon F.} and Neal Fedarko and Hudson, {Sherry A.} and Holly Rohde and Huang, {Shau Ku} and Xu, {Kai Y.}",

year = "2002",

doi = "10.1006/bbrc.2002.6419",

language = "English (US)",

volume = "291",

pages = "111--115",

journal = "Biochemical and Biophysical Research Communications",

issn = "0006-291X",

publisher = "Academic Press Inc.",

number = "1",

}

TY - JOUR

T1 - Cell surface expression of a specific antigenic site on the catalytic subunit of (Na+ + K+)-ATPase

AU - Myers, Allen C.

AU - Bochner, Bruce S.

AU - Tomaselli, Gordon F.

AU - Fedarko, Neal

AU - Hudson, Sherry A.

AU - Rohde, Holly

AU - Huang, Shau Ku

AU - Xu, Kai Y.

PY - 2002

Y1 - 2002

N2 - Structural localization of a peptide region, KRQPRNPKTDKLVNE, in the catalytic subunit of (Na+ + K+)-ATPase was investigated using a specific antibody directed against this peptide in cultured African green monkey kidney CV-1 cells. Immunofluorescence staining of frozen cell sections shows that an anti-KRQPRNPKTDKLVNE antibody (SSA95) interacts with its antigenic site and binds to the extracellular side of the cell membrane. Indirect immunofluorescence and flow cytometric analyses confirmed the presence of this epitope on intact cell surfaces. These results suggest that the KRQPRNPKTDKLVNE region of the (Na+ + K+)-ATPase is expressed on the cellular membrane surface.

AB - Structural localization of a peptide region, KRQPRNPKTDKLVNE, in the catalytic subunit of (Na+ + K+)-ATPase was investigated using a specific antibody directed against this peptide in cultured African green monkey kidney CV-1 cells. Immunofluorescence staining of frozen cell sections shows that an anti-KRQPRNPKTDKLVNE antibody (SSA95) interacts with its antigenic site and binds to the extracellular side of the cell membrane. Indirect immunofluorescence and flow cytometric analyses confirmed the presence of this epitope on intact cell surfaces. These results suggest that the KRQPRNPKTDKLVNE region of the (Na+ + K+)-ATPase is expressed on the cellular membrane surface.

KW - (Na + K)-ATPase

KW - Flow cytometry

KW - Immunofluorescence staining

KW - Positive inotropy

KW - Site-specific antibody

UR - http://www.scopus.com/inward/record.url?scp=0036083180&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036083180&partnerID=8YFLogxK

U2 - 10.1006/bbrc.2002.6419

DO - 10.1006/bbrc.2002.6419

M3 - Article

C2 - 11829469

AN - SCOPUS:0036083180

SN - 0006-291X

VL - 291

SP - 111

EP - 115

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 1

ER -