Cell labeling methods for noninvasive MR imaging of stem cells

Jeff W.M. Bulte

doi:10.1007/978-3-7091-1175-8_5

Cell labeling methods for noninvasive MR imaging of stem cells

Jeff W.M. Bulte

School of Medicine

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Abstract

MRI tracking of stem cells and progenitor cells is now widely being pursued in preclinical models of neurological diseases, including stroke. Typically, the cells of interest are labeled with superparamagnetic iron oxide (SPIO) particles that knock out the signal on the MR images. In this chapter, several methods are described that can be used to achieve intracellular SPIO labeling. In addition, some of the newer approaches are being discussed, in particular the use of perfluorocarbon emulsions for 19F hot spot MRI and MRI reporter genes, with the latter being the much sought-after holy grail for MRI cell tracking.

Original language	English (US)
Title of host publication	Cell-Based Therapies in Stroke
Publisher	Springer-Verlag Wien
Pages	65-74
Number of pages	10
ISBN (Electronic)	9783709111758
ISBN (Print)	9783709111741
DOIs	https://doi.org/10.1007/978-3-7091-1175-8_5
State	Published - Jan 1 2013

ASJC Scopus subject areas

General Medicine

Access to Document

10.1007/978-3-7091-1175-8_5

Cite this

@inbook{a92ffb55f8c04d5e8fd7e923cd639301,

title = "Cell labeling methods for noninvasive MR imaging of stem cells",

abstract = "MRI tracking of stem cells and progenitor cells is now widely being pursued in preclinical models of neurological diseases, including stroke. Typically, the cells of interest are labeled with superparamagnetic iron oxide (SPIO) particles that knock out the signal on the MR images. In this chapter, several methods are described that can be used to achieve intracellular SPIO labeling. In addition, some of the newer approaches are being discussed, in particular the use of perfluorocarbon emulsions for 19F hot spot MRI and MRI reporter genes, with the latter being the much sought-after holy grail for MRI cell tracking.",

author = "Bulte, {Jeff W.M.}",

note = "Publisher Copyright: {\textcopyright} Springer-Verlag Wien 2013.",

year = "2013",

month = jan,

day = "1",

doi = "10.1007/978-3-7091-1175-8_5",

language = "English (US)",

isbn = "9783709111741",

pages = "65--74",

booktitle = "Cell-Based Therapies in Stroke",

publisher = "Springer-Verlag Wien",

}

TY - CHAP

T1 - Cell labeling methods for noninvasive MR imaging of stem cells

AU - Bulte, Jeff W.M.

N1 - Publisher Copyright: © Springer-Verlag Wien 2013.

PY - 2013/1/1

Y1 - 2013/1/1

N2 - MRI tracking of stem cells and progenitor cells is now widely being pursued in preclinical models of neurological diseases, including stroke. Typically, the cells of interest are labeled with superparamagnetic iron oxide (SPIO) particles that knock out the signal on the MR images. In this chapter, several methods are described that can be used to achieve intracellular SPIO labeling. In addition, some of the newer approaches are being discussed, in particular the use of perfluorocarbon emulsions for 19F hot spot MRI and MRI reporter genes, with the latter being the much sought-after holy grail for MRI cell tracking.

AB - MRI tracking of stem cells and progenitor cells is now widely being pursued in preclinical models of neurological diseases, including stroke. Typically, the cells of interest are labeled with superparamagnetic iron oxide (SPIO) particles that knock out the signal on the MR images. In this chapter, several methods are described that can be used to achieve intracellular SPIO labeling. In addition, some of the newer approaches are being discussed, in particular the use of perfluorocarbon emulsions for 19F hot spot MRI and MRI reporter genes, with the latter being the much sought-after holy grail for MRI cell tracking.

UR - http://www.scopus.com/inward/record.url?scp=84956742406&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84956742406&partnerID=8YFLogxK

U2 - 10.1007/978-3-7091-1175-8_5

DO - 10.1007/978-3-7091-1175-8_5

M3 - Chapter

AN - SCOPUS:84956742406

SN - 9783709111741

SP - 65

EP - 74

BT - Cell-Based Therapies in Stroke

PB - Springer-Verlag Wien

ER -

Cell labeling methods for noninvasive MR imaging of stem cells

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this