Cell fusion assays for yeast mating pairs

Eric Grote

doi:10.1007/978-1-59745-250-2_10

Cell fusion assays for yeast mating pairs

Eric Grote

School of Medicine

Research output: Chapter in Book/Report/Conference proceeding › Chapter

14 Scopus citations

Abstract

Yeast mating provides an accessible genetic system for the discovery of fundamental mechanisms in eukaryotic cell fusion. Although aspects of yeast mating related to pheromone signaling and polarized growth have been intensively investigated, fusion itself is poorly understood. This chapter describes methods for measuring the overall efficiency of yeast cell fusion and for monitoring various stages of the fusion process including cell wall remodeling, plasma membrane fusion, and nuclear fusion.

Original language	English (US)
Title of host publication	Cell Fusion
Subtitle of host publication	Overviews and Methods
Publisher	Humana Press
Pages	165-196
Number of pages	32
ISBN (Print)	9781588299116
DOIs	https://doi.org/10.1007/978-1-59745-250-2_10
State	Published - Jan 1 2008

Publication series

Name	Methods in Molecular Biology
Volume	475
ISSN (Print)	1064-3745

Keywords

Cell fusion
Cell wall remodeling
Karyogamy
Mating
Membrane fusion

ASJC Scopus subject areas

Molecular Biology
Genetics

Access to Document

10.1007/978-1-59745-250-2_10

Cite this

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title = "Cell fusion assays for yeast mating pairs",

abstract = "Yeast mating provides an accessible genetic system for the discovery of fundamental mechanisms in eukaryotic cell fusion. Although aspects of yeast mating related to pheromone signaling and polarized growth have been intensively investigated, fusion itself is poorly understood. This chapter describes methods for measuring the overall efficiency of yeast cell fusion and for monitoring various stages of the fusion process including cell wall remodeling, plasma membrane fusion, and nuclear fusion.",

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KW - Karyogamy

KW - Mating

KW - Membrane fusion

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ER -

Cell fusion assays for yeast mating pairs

Abstract

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Keywords

ASJC Scopus subject areas

Access to Document

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