cDNA Cloning of an orphan opiate receptor gene family member and its splice variant

Jia Bei Wang, Peter S. Johnson, Yasuo Imai, Antonio M. Persico, Bradley A. Ozenberger, C. Mark Eppler, George R. Uhl

Research output: Contribution to journalArticlepeer-review

359 Scopus citations


Radioligand binding and cDNA homology studies have suggested the existence of opiate receptors distinct from the recently-cloned, μ, δ and κ receptors. XOR1S, a rat brain cDNA whose predicted translation product displays 67-72% homology with those encoded by μ1, δ1 and κ1 opiate receptor cDNAs, was constructed from two partial cDNAs identified through cDNA homology approaches. A longer XOR1L variant of this cDNA was also identified by polymerase chain reaction studies using genomic DNA and cDNA from brain and peripheral tissues. XOR1 mRNA is most highly expressed in hypothalamus. COS cell expression of both clones confers neither robust binding of opiate ligands nor reproducible opiate inhibition of forskolin-stimulated adenylate cyclase. These studies identify an orphan clone that helps to define features of the opiate receptor gene family, including apparent differential splicing and expression in peripheral tissues.

Original languageEnglish (US)
Pages (from-to)75-79
Number of pages5
JournalFEBS Letters
Issue number1
StatePublished - Jul 4 1994


  • Alternative splicing
  • G-linked receptor
  • Hypothalamus
  • Morphine
  • Seven transmembrane domain

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology


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