cDNA clones encoding IgE-binding factors from a rat-mouse T-cell hybridoma

C. L. Martens, T. F. Huff, P. Jardieu, M. L. Trounstine, R. L. Coffman, K. Ishizaka, K. W. Moore

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cDNA clones encoding rodent IgE-binding factors (IgE-BF) were isolated from cDNA libraries of a rat-mouse T hybridoma that secretes IgE-suppressive factor (IgE-SF) upon incubation with rat IgE. COS7 cells transfected with two of the cDNAs expressed IgE-BF, which selectively potentiate an in vitro IgE response. IgE-BF expressed in COS7 cells are glycoproteins of ~60 and ~11 kDa. DNA sequence analysis of an IgE-BF cDNA revealed a 556-amino acid (62 kDa) protein coding region. The results suggest that IgE-potentiating and IgE-suppressive factors share common precursor polypeptides and that the 11-kDa IgE-BF is derived from a 60-kDa precursor.

Original languageEnglish (US)
Pages (from-to)2460-2464
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Issue number8
StatePublished - 1985
Externally publishedYes

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